TY - JOUR JF - sjimu JO - J. Ilam Uni. Med. Sci. VL - 21 IS - 5 PY - 2013 Y1 - 2013/10/01 TI - Survey and Comparison of Immunization Scale of The Recombinant Proteins of Attachment Subunit of Tetanus and Botulinom(A) Toxins TT - بررسی و مقایسه میزان ایمنی زایی پروتئین های نوترکیب زیر واحد اتصالی توکسین های کزاز و بوتولینیوم)تایپ (A N2 - Introduction: The only presented vaccine against tetanospasmin (the main factor of the fatal disease tetanus) is its toxoid which creates a 10-year period immunization. Ho-wever, the botulinum toxoid (the main factor of the fetal disease botulism) only creates a 2-year period immunization. The-se two toxins have 36% homology and are falling into one family but are different in immunological memory, the scale of hom-oral immunity response and probably the scale of antibody production. The goal of this study was to compare the immunization and antibody titer of fragment C of both tetanus toxin (THc) and botulinom type A (BONT/A-Hc). Materials & Methods: Expression of the recombinant proteins, THc and BONT/A-Hc, was accomplished by using of the tran-sgenic host, E.coli Bl21 DE3 and the pET28a vectors which contained the two genes in an optimum condition. Both the mentioned proteins were derived and puri-fied from the solution phase of cell extract and were evaluated on SDS-PAGE gel. Finally, the antibody titer containing in the serum of immunized animals with each of the aforementioned proteins, was inv-estig-ated and compared by immunoassay test. Findings: The result of SDS-PAGE gel evaluation showed a proper expression and purification of both the recombinant prot-eins, THc and BONT/A-Hc, in solution phase of cell extract. The immunoassay res-ults of serum showed that the antibody titer against the recombinant protein of THc was more than those for the recombinant protein of BONT/A-Hc. Discussion & Conclusion: It is conceived that the significant differences in the antib-ody titer can be related to the longevity of memory cells. However, the result needs more studies. SP - 109 EP - 114 AU - rezaee, e AU - miri, e AU - salimian, j AU - olad, gh AU - sadati, m AU - ebrahimi, m AU - bostani, h AD - KW - antibody titer KW - immunization KW - memory cell KW - tetanus toxin KW - botulinom tox-in UR - http://sjimu.medilam.ac.ir/article-1-1319-en.html ER -