RT - Journal Article T1 - The histological effects of chronic multiple sequential stress on rat testis JF - sjimu YR - 2013 JO - sjimu VO - 21 IS - 3 UR - http://sjimu.medilam.ac.ir/article-1-714-en.html SP - 83 EP - 90 K1 - chronic multiple sequential stress K1 - seminiferous tubule K1 - spermatogonia K1 - sperm K1 - leydig K1 - rat AB - Background: The increase of stress following the technological improvements appears to be an important factor that causes organs disorders like genital system. Therefore the present study was conducted to investigate the effects of chronic multiple sequential stress on rat testis Methods: 18 Wistar rats were divided randomly into two equal groups. In animals under stress, the mice were exposed to different multiple sequential stress as Forced swimming, Restraint, Water deprivation, Isolation and Food deprivation for 10 days while the animals in control group were kept in their cages without any disorders. After weighing the animals and anesthesing with xylazine-Ketamine, the testis of animals were removed and weighed. After fixation with formaldehyde (10%), testis samples were prepared for light microscopic study. The number of spermatogonia, spermatocytes I & II, sperm & leydig cells, thickness of the seminiferous tubule determined using Image Tool software in the studied groups. Finally the data were compared statistically. Results: The present study showed that the mean number of spermatogoniaĜŒ spermatocyte I & IIĜŒ sperm & leydig cells in stress group have significantly decreased compared to control group (P LA eng UL http://sjimu.medilam.ac.ir/article-1-714-en.html M3 ER -