@ARTICLE{Vasoghi, author = {vasoghi, p and ahmadi, n and shahani, m and bahadori monfared, e and azizi, m and }, title = {Evaluating Human Serum Albumin Structure and Stability in the Presence of DTAB}, volume = {21}, number = {7}, abstract ={Introduction: Human serum albumin with its singular structure acts as a carrier mo-lecule that can attach to a variety of sub-stances. Any structural or functional chan-ges in albumin are accompanied with unde-sirable outcomes. In the present research, attachment of some ligands such as det-ergents (DTAB) and sugars (glucose) to albumin were evaluated at temperatures 27°C and 42°C. Materials & Methods: Tris-buffer solusion for albumin with a pH close to the pH of body was prepared. To denature albumin, hydrochloric acid was used. UV spectero-photometric technique was applied to eval-uate denaturation process. Findings: With increment of DTAB conc-entration the maximum optical absorption of albumin decreased. Glucose concent-ration at 400mg/dl level caused a profound change in the optical absorption of albumin that might affect the structure of the mo-lecule. Discussion & Conclusion: Glucose elevate-on has deleterious effects and deteriorates the function of many molecules in blood. The effects may exert on hormones, drug transportation, necessary ions and elements }, URL = {http://sjimu.medilam.ac.ir/article-1-1596-en.html}, eprint = {http://sjimu.medilam.ac.ir/article-1-1596-en.pdf}, journal = {Journal of Ilam University of Medical Sciences}, doi = {}, year = {2014} }