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:: Volume 31, Issue 1 (4-2023) ::
Journal of Ilam University of Medical Sciences 2023, 31(1): 19-28 Back to browse issues page
Synthesis of Titanium Oxide Nanoparticles Functionalized by Glutamine and Conjugated to Thiosemicarbazide and their Effect on the Expression of the CASP3, Bcl2, and BAX genes
Shahrzad Sadat Shahmoradi1 , Ali Salehzadeh * 2, Najmeh Ranji1 , Hadi Habibbollahi1
1- Dept of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
2- Dept of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran , salehzadehmb@yahoo.com
Abstract:   (519 Views)
Introduction: The increasing trend of cancer morbidity and mortality is a major human health concern, indicating the necessity for the design and introduction of novel anticancer compounds. The use of nanotechnology products is a new approach to cancer treatment. Therefore, the current study was performed to synthesize Titanium Dioxide nanoparticles (NPs) functionalized with glutamine and conjugated to Thiosemicarbazide (TiO2@Gln-TSC) to inhibit the proliferation of liver cancer cells line (HepG2) and evaluate the effect of TiO2@Gln-TSC NPs on the expression of apoptotic genes.
Material & Methods: TiO2@Gln-TSC NPs were synthesized by a chemical method and characterized by Fourier-transform infrared spectroscopy (FT-IR), Energy-dispersive X-ray spectroscopy (EDS), Scanning electron microscopy (SEM), and Transmission electron microscopy (TEM). The cytotoxicity of the Titanium Dioxide nanoparticles was evaluated by the MTT assay, and relative gene expression was studied by Real Time PCR method.
Findings: The results showed that the synthesized Titanium Dioxide nanoparticles were spherical with a size range of 59 to 82 nm. The particles had a considerable anti-proliferative effect on liver cancer cells line with IC50 of 80 µg/mL. The treatment of the cancer cell line with TiO2@Gln-TSC NPs significantly increased the expression of the CASP3, BAX, and BCL2 by 2.8, 2.7, and 1.3 folds, respectively, which indicated the activation of apoptotic pathways in the treated cells.
Discussion & Conclusion: The results showed that the TiO2@Gln-TSC NPs could inhibit the proliferation of liver cancer cells line and by triggering the apoptosis pathway.

 
Keywords: Apoptosis, Caspase, Liver cancer, Thisemicarbazide
Full-Text [PDF 939 kb]   (270 Downloads)    
Type of Study: Research | Subject: biology
Received: 2022/06/28 | Accepted: 2022/10/25 | Published: 2023/04/4
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Shahmoradi S S, Salehzadeh A, Ranji N, Habibbollahi H. Synthesis of Titanium Oxide Nanoparticles Functionalized by Glutamine and Conjugated to Thiosemicarbazide and their Effect on the Expression of the CASP3, Bcl2, and BAX genes. J. Ilam Uni. Med. Sci. 2023; 31 (1) :19-28
URL: http://sjimu.medilam.ac.ir/article-1-7631-en.html


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Volume 31, Issue 1 (4-2023) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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