:: Volume 30, Issue 6 (2-2023) ::
Journal of Ilam University of Medical Sciences 2023, 30(6): 9-20 Back to browse issues page
Synthesis of Silver Nanoparticles using Methanol Extract of Bunium Persicum and the Evaluation of its Cytotoxic, Antileishmanial, and Antimicrobial Activities
Fatemeh Sharifi1 , Neda MohamadI2 , Sara Soltanian * 3, Mohsen Doostmohammadi4
1- Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
2- Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran
3- Dept of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran , genetic1359@yahoo.com
4- Pharmaceutic Research Center, Institute of Neuropharmacology, Kerman University of Medical Science, Kerman, Iran
Abstract:   (814 Views)
Introduction: The unique properties of silver nanoparticles (Ag-NPs) produced using plant extract make them attractive for use in medical and industrial applications. Bunium persicum from the Apiaceae family is native to Iran, Afghanistan, Pakistan, and some Central Asian countries, which is locally known as "Kermanin Black Cumin" in Iran. In this study, Ag-NPs were synthesized using methanol extract of B. persicum as the regenerating and stabilizing agent for the first time and were followed by the characterization and evaluation of its biological potency.
Material & Methods: Synthesis of Ag-NPs was conducted using the B. persicum extract. Ultraviolet-visible (UV-VIS) spectroscopy was used to detect the presence of nanoparticles. Scanning Electron Microscope (SEM) was also employed to visualize the surface morphology, shape, and size distribution of nanoparticles. Fourier Transform Infrared Spectroscopy (FTIR) is very sensitive to the chemical surface of nanoparticles and was utilized to identify functional groups in the nanoparticles. Cytotoxicity against cancer cell lines and antileishmanial activities were investigated using MTT assay, and the well diffusion method was used to detect the antibacterial property of the synthesized nanoparticles.
Findings: UV-VIS spectrum exhibits an absorption band at around 400-450 nm suggesting the formation of biological Ag-NPs. The size and morphological properties of nanoparticles were assessed by SEM which showed that particles have spherical shapes with a diameter of about 20-70 nm. Ag-NPs showed cytotoxicity against human glioblastoma cancer cell line A-172 (IC50:7.2 µg/ml) and breast cancer cell line MCF-7 (IC50:7.6 µg/ml) after 48 h treatment. Ag-NPs presented antimicrobial activity against Gram-positive and Gram-negative bacteria. The present study confirmed good antileishmanial activity against the promastigote and amastigote stages of Leishmania major. The IC50 values of Ag-NPs and Glucantime® were 73.89 and 16.17μg/mL for promastigote, as well as 171.02 and 398.21 μg/mL for amastigotes assays, respectively.
Discussion & Conclusion: The extract of B. persicum has the ability to reduce Ag+ ions to Ag nanoparticles. Moreover, the fabricated Ag-NPs have good cytotoxicity, antibacterial, and antileishmanial activities.
 
Keywords: Antibacterial activity, Antileishmanial effects, Bunium persicum, Cytotoxicity, Green synthesis, Silver nanoparticles
Full-Text [PDF 1021 kb]   (336 Downloads)    
Type of Study: Research | Subject: biology
Received: 2022/01/3 | Accepted: 2022/04/19 | Published: 2023/02/4
References
1. Dipankar C, Murugan S. The green synthesis, characterization and evaluation of the biological activities of silver nanoparticles synthesized from Iresine herbstii leaf aqueous extracts. Colloids Surf B Biointerfaces 2012; 98: 112-19. doi: 10.1016/j.colsurfb.2012.04.006.
2. Sharifi F, Sharififar F, Soltanian S, Doost-mohammadi M, Mohamadi. Synthesis of silver nanoparticles using Salvia officinalis extract: Structural characterization, cyto-toxicity, antileishmanial and antimicrobial activity. Nanomed Res J 2020; 5: 339-46. doi: 10.22034/nmrj2020.04.005.
3. Gour A, Jain NK. Advances in green synthesis of nanoparticles. Artif Cells Nanomed Biotechnol 2019; 47: 844-51. doi: 10.1080/21691401.2019.1577878.
4. Nisar P, Ali N, Rahman L, Ali M, Shinwari ZK. Antimicrobial activities of biologically synthesized metal nanoparticles: an insight into the mechanism of action. J Biol Inorg Chem 2019; 24: 929-41. doi: 10.1007/s00775-019-01717.
5. Soltanian S, Sheikhbahaei M, Mohamadi N. Cytotoxicity evaluation of methanol extracts of some medicinal plants on P19 embryonal carcinoma cells. J Appl Pharm Sci 2017; 7: 142-9. doi: 0.7324/JAPS.2017.70722.
6. Karimzadeh MR, Soltanian S, Sheikhbahaei M, Mohamadi N. Characterization and biological activities of synthesized zinc oxide nanoparticles using the extract of Acan-tholimon serotinumn. Green Process Synth 2020; 9: 722-33. doi: org/10.1515/gps-2020-0058.
7. Soltanian S, Sheikhbahaei M, Mohamadi N, Pabarja A, Fekri Soofi Abadi M, Mohammadi Tahroudi MH. Biosynthesis of zinc oxide nanoparticles using hertia intermedia and evaluation of its cytotoxic and antimicrobial activities. Bio Nano Sci 2021; 11:245-55. doi: org/10.1007/s12668-020-00816-z.
8. Kunzemann J, Herrmann K. Isolation and identification of flavon (ol)-O-glycosides in caraway (Carum carvi L.), fennel (Foeniculum vulgare Mill.), anise (Pimpinella anisum L.), and coriander (Coriandrum sativum L.), and of flavon-C-glycosides in anise. I. Phenolics of spices (author's transl). Z Lebensm Unters Forsch 1977; 164: 194-200. doi: 10.1007/BF01263030.
9. Sekine T, Sugano M, Majid A, Fujii Y. Antifungal effects of volatile compounds from black zira (Bunium persicum) and other spices and herbs. J Chem Ecol 2007; 33: 2123-32. doi: 0.1007/s10886-007-9374-2.
10. Hassanzad Azar H, Taami B, Aminzare M, Daneshamooz S. Bunium persicum (Boiss.) B. Fedtsch: An overview on Phytochemistry, Therapeutic uses and its application in the food industry. J Appl Pharm Sci 2018; 8: 150-58. doi:10.7324/JAPS.2018.81019.
11. Khan I, Bawazeer S, Rauf A, Qureshi MN, Muhammad N, Al-Awthan YS, et al. Synthesis, biological investigation and catalytic application using the alcoholic extract of Black Cumin (Bunium Persicum) seeds-based silver nanoparticles. J Nanostructure Chem 2022; 12: 59-77. doi: 10.1007/s40097-021-00402-z.
12. Bamorovat M, Sharifi I, Aflatoonian MR, Sharifi H, Karamoozian A, Sharifi F, et al. Risk factors for anthroponotic cutaneous leishmaniasis in unresponsive and responsive patients in a major focus, southeast of Iran. PloS one 2018; 13: e0192236. doi: 10.1371/journal.pntd.0000639.
13. Vivek R, Thangam R, Muthuchelian K, Gunasekaran P, Kaveri K, Kannan S. Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells. Process Biochem 2012; 47: 2405-10. doi: 10.1016/
14. j.procbio.2012.09.025.
15. Mohanta YK, Panda SK, Jayabalan R, Sharma N, Bastia AK, Mohanta TK. Antimicrobial, antioxidant and cytotoxic activity of silver nanoparticles synthesized by leaf extract of Erythrina suberosa (Roxb.). Antimicrobial, antioxidant and cytotoxic activity of silver nanoparticles synthesized by leaf extract of Erythrina suberosa (Roxb.) Front Mol Biosci 2017; 4: 14. doi: 10.3389/fmolb.2017.00014/full.
16. Soltanian S, Mahboubeh S, Fatemeh S, Mohamadi N. Synthesis and Biological Characterization of Silver Nanoparticles Biosynthesized by Semenovia suffruticosa. J Nano Res 2021; 66: 45-60. doi: 10.4028/www.scientific.net/JNanoR.66.45.
17. Zhang G, Gurtu V, Kain SR, Yan G. Early detection of apoptosis using a fluorescent conjugate of annexin V. Biotechniques 1997; 23 (3): 525-531. doi: 10.2144/97233pf01.
18. Kajani AA, Zarkesh-Esfahani SH, Bordbar A-K, Khosropour AR, Razmjou A, Kardi M. Anticancer effects of silver nanoparticles encapsulated by Taxus baccata extracts. J Mol Liq 2016; 223: 549-56. doi: 10.2144/97233pf01.
19. Sivanandam V, Purushothaman M, Karunanithi M. Green synthesis of silver nanoparticles using plant leaf extract and evaluation of their antibacterial and in vitro antioxidant activity. Asian Pac J Trop Biomed 2020; 6: 100086. doi: 10.1016/j.rinma.2020.100086.
20. Ghanbar F, Mirzaie A, Ashrafi F, Noorbazargan H, Jalali MD, Salehi S, et al. Antioxidant, antibacterial and anticancer properties of phyto-synthesised Artemisia quttensis Podlech extract mediated AgNPs. IET nanobiotechnol 2017; 11: 485-92. doi: 10.1049/iet-nbt.2016.0101.
21. Gao X, Yourick JJ, Topping VD, Black T, Olejnik N, Keltner Z, et al. Toxicogenomic study in rat thymus of F1 generation offspring following maternal exposure to silver ion. Toxicol rep 2015; 2: 341-50. doi: 10.1016/j.toxrep.2014.12.008.
22. Feng QL, Wu J, Chen G, Cui F, Kim T, Kim J. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 2000; 52: 662-8. doi: 10.1002/1097-4636(20001215)52:4<662::AID-JBM10>3.0.
23. CO;2-3.
24. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology 2005; 16: 2346. doi: 10.1088/0957-4484/16/10/059/meta.
25. Drlica K, Zhao X. DNA gyrase, topoisomerase IV, and the 4-quinolones. Microbiol Mol Biol Rev 1997; 61: 377. doi: 10.1128/mmbr.61.3.377-392.1997.
26. Black B, Beadle K, Harding SJ. Out of sight, out of mind: Impact of an antimicrobia stewardshipinitiative to reduce fluoroquinolone utilization. J Clin Pharm Ther 2022; 5: 668-73.doi: doi.org/10.1002/jac5.1658.
27. Liu X, Huang L, Zhu W, Yuan P, Wan R, et al. Fluoroquinolones increase the risk of serious arrhythmias: a systematic review and meta-analysis. Medicine 2017; 96 :44. doi: 10.1097/MD.0000000000008273.
28. Salayová A, Bedlovičová Z, Daneu N, Baláž M, Lukáčová Bujňáková Z, Balážová Ľ, et al. Green synthesis of silver nanoparticles with antibacterial activity using various medicinal plant extracts: Morphology and antibacterial efficacy. Nanomaterials 2021; 11:1005. doi:10.3390/nano11041005.
29. Loo YY, Rukayadi Y, Nor-Khaizura MAR, Kuan CH, Chieng BW, Nishibuchi M, et al. In vitro antimicrobial activity of green synthesized silver nanoparticles against selected gram-negative foodborne pathogens. Front microbiol 2018; 9: 1555. doi: 10.3389/fmicb.2018.
30. Foroutan M, Khademvatan S, Majidiani H, Khalkhali H, Hedayati-Rad F, Khashaveh S, et al. Prevalence of Leishmania species in rodents: a systematic review and meta-analysis in Iran. Acta tropica 2017; 172: 164-72. doi: 10.1016/j.actatropica.2017.04.022.
31. Piroozi B, Moradi G, Alinia C, Mohamadi P, Gouya MM, Nabavi M, et al. Incidence, burden, and trend of cutaneous leishmaniasis over four decades in Iran. Iran J Public Health 2019; 48: 28-35.
32. Barati M, Sharifi I, Sharififar F, Hakimi Parizi M, Shokri A. Anti-leishmanial activity of gossypium hirsutum L. Ferula assa-foetida L. and Artemisia aucheri Boiss. Extracts by colorimetric assay. Antiinfec Agents 2014; 12: 159-164.
33. Barati M, Sharifi L,Sharififar F. Antileishmanial activity of Artemisia aucheri, Ferula asafoetid and Gossypium hirsutum extracts on Leishmania major promastigotes in vitro. Ann Military Health Sci Res 2011; 8:166-72.

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