:: Volume 30, Issue 4 (10-2022) ::
Journal of Ilam University of Medical Sciences 2022, 30(4): 56-65 Back to browse issues page
Antibacterial Effect of Arginine, Protamine, Aqueous Extracts of Green Tea, and Aloe Vera against Escherichia Coli
Sanaz Najafi1 , Abolfazl Davoodabadi2 , Sohrab Kazemi3 , Maryam Ghasempour * 4
1- Student Research Committee, Babol University of Medical Sciences, Babol, Iran
2- Infection Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
3- Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
4- Oral Health Research Center, Institute of Health, Babol University of Medical Sciences, Babol, Iran , ma_ghasempour_ir@yahoo.com
Abstract:   (1000 Views)
Introduction: E. coli is one of the failure factors in the pulpectomy of primary and permanent teeth. It is also responsible for many infectious diseases in Humans. This study aimed to determine antibacterial effect of arginine, protamine, aqueous extract of green tea, and Aloe vera against Escherichia coli (E. coli).
Material & Methods: Aqueous extract of green tea, Aloe vera, and protamine with a concentration of 400 mg/ml, and arginine at a concentration of 160 mg/ml were used in this experimental study. E. coli was cultured on Mueller-Hinton broth and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the test materials against this microorganism was determined using micro-dilution test. Sodium hypochlorite 2.5% was used as a positive control.
(Ethic code: 9643822)
Findings: It was found that protamine had the highest antibacterial activity (with MIC and MBC 0.62 mg/mL) and aqueous extract of green tea showed the lowest antibacterial activity against E. coli (with MIC100 mg/ml and MBC 200 mg/ml). Positive control (sodium hypochlorite 2.5%) showed better antibacterial activity against E. coli compared to other test materials.
Discussion & Conclusion: Apart from sodium hypochlorite, among the tested materials, protamine had the maximum effect and aqueous extract of aloe vera, arginine, and aqueous extract of green tea showed the lowest antibacterial effect against E. coli., respectively.
 
Keywords: Aloe vera, Arginine, Escherichia Coli, Protamine, Tea
Full-Text [PDF 684 kb]   (701 Downloads)    
Type of Study: Research | Subject: Bacteriology
Received: 2021/12/31 | Accepted: 2022/05/28 | Published: 2022/10/7
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Ethics code: 9643822

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