:: Volume 30, Issue 5 (12-2022) ::
Journal of Ilam University of Medical Sciences 2022, 30(5): 32-39 Back to browse issues page
Effect of Salix Alba Leaves Hydro-Alcoholic Extract on PTZ-Induced Seizure in Male Mice
Fakhri Khalighi Sigaroudi1 , Mohammad Rostampour * 2, Bahram Soltani tehrani3
1- Research Student Committee, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
2- Cellular and Molecular Research Center, Facultyof Medicine, Guilan University of Medical Sciences, Rasht, Iran , rostampour@gums.ac.ir
3- Dept of Pharmacology, Faculty of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
Abstract:   (701 Views)
Introduction: Regarding the chronic nature of epilepsy and resistance to chemical drugs, herbal medicine has received remarkable interest. This study investiagted the anticonvulsant effects of hydro-alcoholic extract of Salix Alba leaves on PTZ-induced seizure in male mice.
Material & Methods: In total, 56 albino male mice weighing 20-25 g were divided randomly into seven groups (8 animals per group) including negative (saline), positive (Phenobarbital) control, and five groups which received different doses of hydro-alcoholic extract of Salix Alba leaves. After 45 min, all groups received the convulsant pentylene tetrazole, and the onset time of myoclonic, tonic-clonic seizures, and the 24-hour mortality rate were measured.
(Ethic code: IR.GUMS.REC.1394.566)
Findings: The extract significantly delayed the onset of myoclonic and tonic-clonic seizures at a dose of 100 mg/kg (P<0.01) and for other doses (P<0.001), compared to the control group. Moreover, the extract decreased the 24-h death. This was significant at 1000 mg/kg (P<0.001), as well as 300, 500, and 800 mg/kg (P<0.05), compared to the control group.
Discussion & Conclusion: It seems that the hydro-alcoholic extract of Salix Alba leaves has a significant diminishing effect on PTZ-induced seizures in male mice which is due to its anti-inflammatory and anti-oxidant effects.
Keywords: Mice, Pentylenetetrazol, Salix Alba leaves, Seizure
Full-Text [PDF 706 kb]   (296 Downloads)    
Type of Study: Research | Subject: physiology
Received: 2021/11/19 | Accepted: 2022/05/15 | Published: 2022/12/6
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Ethics code: IR.GUMS.REC.1394.566

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