:: Volume 29, Issue 6 (1-2022) ::
Journal of Ilam University of Medical Sciences 2022, 29(6): 81-89 Back to browse issues page
Effect of Continuous Aerobic Training with Citrus Aurantium L. on Mitogen-Activated Protein Kinase and Phosphatidylinositol 3-Kinases Gene Expression in the Liver Tissue of the Elderly Rats
Zinab Shykholeslami1 , Ahmad Abdi * 2, Seyed Ali Hosseini3 , Alireza Barari1
1- Dept of Sports Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Dept of Sports Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , a.abdi58@gmail.com
3- Dept of Sports Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
Abstract:   (1346 Views)
Introduction: Aging impairs the function of genes involved in hepatic metabolisms, such as Mitogen-activated protein kinase (AMPK) and Phosphatidylinositol 3-kinases (PI3K). Exercise and Citrus Aurantium (CA) have a beneficial effect on improving metabolic factors in aging. This study aimed to investigate the effect of aerobic training (T) with consumption of CA on the AMPK and PI3K gene expression levels in the liver tissue of elderly rats.
Material & Methods: In this experimental study, 25 elderly female rats were divided into five groups of five animals per group. The groups included 1) control, 2) sham, 3) CA, 4) T, and 5) T+CA. Over the course of eight weeks, the T groups ran on the treadmill three sessions per week at 65% to 75% of the maximum running speed for 15 min in the first week to 31 min in the eighth week. The supplement groups also received 300 mg/kg/day of CA extract peritoneal. After 48 h of the last training session and CA consumption, AMPK and PI3K gene expression levels in the liver tissue were measured by the real-time PCR method.
(Ethic code: IR.IAU.M.REC.1399.032)
Findings: AMPK expression was significantly increased in CA (P=0.043), T (P=0.025,) and T+CA (P=0.009) groups, compared to the control group. Moreover, a significant increase in the PI3K expression was observed in the T+CA (P=0.031) group, compared to the control group.
Discussion & Conclusion: The results of the present study showed that T and CA might improve liver cell function in elderly rats by increasing the expression of AMPK and PI3K genes.
Keywords: AMPK, Citrus aurantium, Liver metabolism, PI3K
Full-Text [PDF 604 kb]   (527 Downloads)    
Type of Study: Research | Subject: Physical Education
Received: 2021/02/22 | Accepted: 2021/08/28 | Published: 2022/02/4
1. Collblack S, Bhushan A, Fritsch K. Integrating poverty and gender into health programs: a sourcebook for health professionals. Nurs Health Sci 2007;9:246-53. doi.10.1111/j.1442-2018.2007.00340.x.
2. Sharifzadeh G, Moodi M, Akhbari H. [Investigating health status of older people supported by Imam]. Iranian J Age2010;5:52-59. (Persian)
3. Dzamko N, van Denderen BJ, Hevener AL, Jorgensen SB, Honeyman J, Galic S, et al. AMPK β1 deletion reduces appetite, preventing obesity and hepatic insulin resistance. J Biol Chem 2010;285:115-22. doi.10.1074/jbc.M109.056762.
4. Tao R, Gong J, Luo X, Zang M, Guo W, Wen R, et al. AMPK exerts dual regulatory effects on the PI3K pathway. J Mol Sig 2010;5:1-9. doi.10.1186/1750-2187-5-1.
5. Bitto A, Wang AM, Bennett CF, Kaeberlein M. Biochemical genetic pathways that modulate aging in multiple species. Cold Spr Harb Pers Med 2015;5:25114. doi.10.1101/cshperspect.a025114.
6. Reznick RM, Zong H, Li J, Morino K, Moore IK, Hannah JY, et al. Aging associated reductions in AMP-activated protein kinase activity and mitochondrial biogenesis. Cell Metab 2007;5:151-6. doi.10.1016/j.cmet.2007.01.008.
7. Mulligan JD, Gonzalez AA, Kumar R, Davis AJ, Saupe KW. Aging elevates basal adenosine monophosphate activated protein kinase activity and eliminates hypoxic activation of AMPK in mouse liver. J Gerontol A Biol Sci Med Sci 2005;60:21-7. doi.10.1093/gerona/60.1.21.
8. Wasityastuti W, Habib NA, Sari DC, Arfian N. Effects of low and moderate treadmill exercise on liver of d‐galactose‐exposed aging Rat model Physiol Rep2019;7:14279. doi.10.14814/phy2.14279.
9. Yi X, Cao S, Chang B, Zhao D, Gao H, Wan Y, et al. Effects of acute exercise and chronic exercise on the liver leptin Ampk Acc signaling pathway in rats with type 2 diabetes. J Diabete Res2013;2013. doi.10.1155/2013/946432. Epub 2013 Dec 17.
10. Upchurch DM, Chyu L. Use of complementary and alternative medicine among American women. Womens Health Issues2005;15:5-13. doi.10.1016/j.whi.2004.08.010.
11. Suntar I, Khan H, Patel S, Celano R, Rastrelli L. An overview on Citrus aurantium L. its functions as food ingredient and therapeutic agent. Oxid Med Cell Long2018;2018:7864269. doi.10.1155/2018/786426.
12. Yazdanparast Chaharmahali B, Azarbayjani MA, Peeri M, Farzanegi Arkhazloo P. The effect of moderate and high intensity interval trainings on cardiac apoptosis in the old female Rats. Rep Heal Care2018;4:26-35.
13. He W, Li Y, Liu M, Yu H, Chen Q, Chen Y, et al. Citrus aurantium L. and its flavonoids regulate TNBS induced inflammatory bowel disease through anti inflammation and suppressing isolated jejunum contraction. Int J Mol Sci. 2018;19:3057. doi.10.3390/ijms19103057.
14. Canto C, Jiang LQ, Deshmukh AS, Mataki C, Coste A, Lagouge M, et al. Interdependence of AMPK and SIRT1 for metabolic adaptation to fasting and exercise in skeletal muscle. Cell Metab 2010;11:213-9. doi.10.1016/j.cmet.2010.02.006.
15. Marosi K, Bori Z, Hart N, Sarga L, Koltai E, Radak Z, et al. Long term exercise treatment reduces oxidative stress in the hippocampus of aging Rats. Neuroscience2012;226:21-8. doi. 10.1016/j.neuroscience.2012.09.001.
16. Nikroo H, Nematy M, Sima H, AttarzadeHosseini S, Pezeshki M, Esmaeilzadeh A, et al. [Therapeutic effects of aerobic exercise and low calorie diet on nonalcoholic steatohepatitis]. Govaresh 2013;17:245-53. (Persian)
17. Cao S, Li B, Yi X, Chang B, Zhu B, Lian Z, et al. Effects of exercise on AMPK signaling and downstream components to PI3K in rat with type 2 diabetes. PLos One 2012;7:51709. doi. 10.1371/journal.pone.0051709.
18. Goodyear LJ, Giorgino F, Balon TW, Condorelli G, Smith RJ. Effects of contractile activity on tyrosine phosphoproteins and PI 3-kinase activity in rat skeletal muscle. Am J Physiol 1995;268:987-95. doi.10.1152/ajpendo.1995.268.5.E987.
19. Lin JY, Kuo WW, Baskaran R, Kuo CH, Chen YA, Chen WST, et al. Swimming exercise stimulates Igf1/Pi3k/Akt and Ampk/Sirt1/Pgc1α survival signaling to suppress apoptosis and inflammation in aging hippocampus. Aging 2020;12:6852. doi.10.18632/aging.103046.
20. Sriwijitkamol A, Coletta DK, Wajcberg E, Balbontin GB, Reyna SM, Barrientes J, et al. Effect of acute exercise on Ampk signaling in skeletal muscle of subjects with type 2 diabetes a time course and dose response study. Diabetes 2007;56:836-48. doi.10.2337/db06-1119.
21. Arad M, Seidman CE, Seidman J. AMP activated protein kinase in the heart role during health and disease. Circ Res2007;100:474-88. doi. 10.1161/01.RES.0000258446.23525.37.
22. Funakoshi M, Tsuda M, Muramatsu K, Hatsuda H, Morishita S, Aigaki T. A gain of function screen identifies wdb and lkb1 as lifespan extending genes in Drosophila. Biochem Biophys Res Com 2011;405:667-72. doi.10.1016/j.bbrc.2011.01.090.
23. Viollet B, Andreelli F, Jorgensen SB, Perrin C, Flamez D, Mu J, et al. Physiological role of AMP-activated protein kinase insights from knockout mouse models. Biochem Soc Trans. 2003;31:216-9. doi.10.1042/bst0310216.
24. Richter EA, Ruderman NB. AMPK and the biochemistry of exercise implications for human health and disease. Biochem J 2009;418:261-75. doi.10.1042/BJ20082055.
25. Park J, Kim HL, Jung Y, Ahn KS, Kwak HJ, Um JY. Bitter orange Citrus aurantium Linne improves obesity by regulating adipogenesis and thermogenesis through Ampk activation. Nutrients 2019;11:1988. doi.10.3390/nu11091988.
26. Sui GG, Xiao HB, Lu XY, Sun ZL. Naringin activates Ampk resulting in altered expression of Srebps and Pcsk9 and Ldlr to reduce body weight in obese C57bl/6J Mice. J Agric Food Chem 2018;66:8983-90. doi.10.1021/acs.jafc.8b02696.
27. Hong NY, Cui ZG, Kang HK, Lee DH, Lee YK, Park DB. P-Synephrine stimulates glucose consumption via Ampk in L6 skeletal muscle cells. Biochem Biophys Res Com2012;418:720-4.
28. Choi BK, Kim TW, Lee DR, Jung WH, Lim JH, Jung JY, et al. A polymethoxy flavonoids rich Citrus aurantium extract ameliorates ethanol induced liver injury through modulation of Ampk and Nrf2 related signals in a binge drinking mouse model. Phytotherap Res 2015;29:1577-84. doi.10.1002/ptr.5415.
29. Ramezannezhad P, Heidarisoureshjani S, Suhan T. Protective effects of some medicinal plants against myocardial hypoxia. Int J Biolo Chem 2019;12:112-27. doi.10.26577/ijbch-2019-1-i13.
30. Shykholeslami Z, Abdi A, Barari A, Hosseini SA. The effect of aerobic training with Citrus aurantium L. on Sirt1 and Pgc1α gene expression levels in the liver tissue of elderly Rats. Jorjani Biomed J 2019;7:57-65

Ethics code: IR.IAU.M.REC.1399.032

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