:: Volume 29, Issue 5 (11-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(5): 75-88 Back to browse issues page
Investigation of the Anticancer Effects of Nanocomposite of the Modified Graphene Oxide with Isatin-3-Semicarbazone on the Retinoblastoma Cells (Y79) Invitro
Zahra Ramezani1 , Hasan Tahermansouri * 2, Farhoush Kiani1
1- Dept of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Dept of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , h.tahermansuri@iauamol.ac.ir
Abstract:   (700 Views)
Introduction: The most common ocular tumor is retinoblastoma, which can be treated by different methods. The use of carbon nanostructures has attracted much attention due to their unique properties, such as crossing cell membranes, for cancer diagnosis and other medical applications. This study aimed to investigate and assess the toxicity of the modified graphene oxide (GO) with isatin-3-semicarbazone (ISA) in order to treat retinoblastoma.
Material & Methods: This study utilized ISA to modify the GO. Subsequently, the modified GO-ISA was characterized by different techniques, such as Fourier transform infrared spectrometry, Scanning Electron Microscope, and Thermal Gravimetric Analysis. The cell toxicity of samples was assessed by MTT assay for two types of cells of retinoblastoma (Y79) and adipose-derived stem cells (ADSC) after 24 h at different concentrations. Data were analyzed in SPSS software using one-way analysis of variance (ANOVA). A p-value less than 0.05 was considered statistically significant.
Findings: The results showed that in the concentration range of 10-50 µL, GO-ISA decreased significantly (P<0.01) the conservation percentage of retinoblastoma cells from 73.98% to 27.48%, while these values for ADSC decreased from 78.16% to 50.11% (P<0.01). In addition, GO-ISA at 30 µL resulted in a significant increase (P<0.01) in the cell toxicity of the retinoblastoma (26%), compared to GO.
Discussion & Conclusion: GO-ISA increases the toxicity of the retinoblastoma cells, compared to GO which was significant at 30 µL. Therefore, this modification of the surface can be useful in future applications. With this modification of the surface, the active sites are provided for the biological and pharmacogenetic reactions in future.
Keywords: Graphene oxide, Isatin, Retinoblastoma, Surface modification, Toxicity
Full-Text [PDF 1048 kb]   (259 Downloads)    
Type of Study: Research | Subject: General
Received: 2020/11/15 | Accepted: 2021/06/21 | Published: 2021/11/8
1. Castillo BV, Kaufman L. Pediatric tumors of the eye and orbit. Pediatr Clin North Am 2003; 50: 149-72. doi.10.1016/S0031-3955(02)00115-3
2. Shields CL, Shields JA. Diagnosis and management of retinoblastoma. Cancer Cont2004; 11: 317-27. doi.10.1177/107327480401100506
3. Thampi S, Hetts SW, Cooke DL, Stewart PJ, Robbins E, Banerjee A, et al. Superselective intra arterial melphalan therapy for newly diagnosed and refractory retinoblastoma: results from a single institution. Clin Ophthalmol 2013; 7: 981-9. doi.10.2147/OPTH.S43398
4. Anubhav C, Shashi DS. Chemotherapy in retinoblastoma. Int J Therap Rehabil Res 2016; 5: 55-60. doi. 10.5455/ijtrr.000000116
5. Kooshafar Z, Salimi M, Javid A. [Evaluating antitumor effect of a novel hydrazide derivative in mammalian mice model]. Koomesh 2018; 20: 582-7. (Persian)
6. Yamanaka YJ, Leong KW. Engineering strategies to enhance nanoparticle mediated oral delivery. J Biomate Sci Pol Edu 2008; 19:1549-70. doi. 10.1163/156856208786440479
7. Choi YE, Kwak JW, Park JW. Nanotechnology for early cancer detection. Sensors 2010; 10: 428-55. doi.10.3390/s100100428
8. Yang X, Zhang X, Ma Y, Huang Y, Wang Y, Chen Y. Superparamagnetic graphene oxide Fe3o4 nanoparticles hybrid for controlled targeted drug carriers. J Mate Chem 2009; 19: 2710-4. doi.10.1039/B821416F
9. Sun Z, James DK, Tour JM. Graphene chemistry synthesis and manipulation. J Phys Chem Let 2011; 2: 2425-32. doi.10.1021/jz201000a
10. Papageorgiou DG, Kinloch IA, Young RJ. Mechanical properties of graphene and graphene based nanocomposites. Prog Mate Sci 2017; 90: 75-127. doi.10.1016/j.pmatsci.2017.07.004
11. Wei J, Zang Z, Zhang Y, Wang M, Du J, Tang X. Enhanced performance of light controlled conductive switching in hybrid cuprous oxide reduced graphene oxide Cu2o rgo nanocomposites. Opt Let 2017; 42: 911-4. doi.10.1364/OL.42.000911
12. Lu X, Li L, Song B, Moon K, Hu N, Liao G, et.al. Mechanistic investigation of the graphene functionalization using p-phenylenediamine and its application for supercapacitors. Nano Ene 2015; 17: 160-70. doi.10.1016/j.nanoen.2015.08.011
13. Huang J, Jacobsen J, Larsen SW, Genina N, Weert M, Mullertz A, et al. Graphene oxide as a functional excipient in buccal films for delivery of clotrimazole effect of molecular interactions on drug release and antifungal activity in vitro. Int J Pharm 2020; 589: 119811. doi.10.1016/j.ijpharm.2020.119811
14. Chouhan A, Mungse H P, Khatri OP. Surface chemistry of graphene and graphene oxide a versatile route for their dispersion and tribological applications. Adv Coll Int Sci 2020; 283: 102215. doi.10.1016/j.cis.2020.102215
15. Pandeya SN, Smitha S, Jyoti M, Sridhar SK. Biological activities of isatin and its derivatives. Acta Pharm2005; 55:27-46.
16. Vine KL, Matesic L, Locke JM, Ranson M, Skropeta D. Cytotoxic and anticancer activities of isatin and its derivatives a comprehensive review from 2000-8. Ant Age Med Chem 2009; 9: 397-414.
17. Jordan MA. Mechanism of action of antitumor drugs that interact with microtubules and tubulin. Curr Med Chem Ant Age2002; 2:1-17. doi.10.2174/1568011023354290
18. Shokrzadeh M, Emami S, Amirzadeh M, Modanloo M. [Cytotoxic effects of Dibromoisatin derivatives on hela and hepg2 cell lines using mtt assay]. Mazandaran Uni Med Sci 2017; 27:24-31. (Persian)
19. Emami S, Raeesi M. [Synthesis of Ciprofloxacin isatin conjugates as potential cytotoxic agents]. J Mazandaran Uni Med Sci 2016; 26: 161-9. (Persian)
20. Pandeya SN, Tripathi K, Kulshreshtha S. Synthesis and antifungal activity of isatin3 semicarbazone. Asian J Chem 2009; 21: 3367-70.
21. Haydarikashl S, Entezari M, Hekmati M, Solaimani S. [Functionalization of carboxylated multi walled nanotubes with diazo compounds of aromatic amins and anti cancer study on sw742 cell line by invitro conditions]. J Ilam Uni Med Sci 2013; 21:138-43. (Persian)
22. Haydari kashl S, Tahermansori H, Atghaee M, Biazar E, Saifipour F, Avazpour M, et al. [Evaluation of multi walled carbon nanotubes cytotoxicity elements oxathiazole and phenyl hydrazine derivative was prepared by microwave]. J Ilam Uni Med Sci2013; 20: 255-64. (Persian)
23. Jastrzebska A M, Kurtycz P, Olszyna AR. Recent advances in graphene family materials toxicity investigations. J Nanopart Res 2012; 14:1320. doi.10.1007/s11051-012-1320-8
24. Tahermansouri H, Abedi E. One pot functionalization of short carboxyl multi walled carbon nanotubes with ninhydrin and thiourea via Microwave and thermal methods and their effect on the MKN-45 and MCF7cancer cells. Full Nanotub Carbon Nanostru2014; 22: 834-44. doi.10.1080/1536383X.2012.742428
25. Tahermansouri H, Mirosanloo A. One pot and three component functionalization of short multi walled carbon nanotubes with isatoic anhydride and benzyl amine and their effect on the MKN-45 and MCF7 cancer cells. Fullerenes, Nanotub Carbon Nanostruc 2015; 23: 500-8. doi.10.1080/1536383X.2013.868440
26. Tahermansouri H, Islami F, Gardaneh M, Kiani F. Functionalisation of multiwalled carbon nanotubes with thiazole derivative and their influence on SKBR3 and HEK293 cell lines. Mate Technol 2016; 31: 371-6. doi. 10.1179/1753555715Y.0000000062
27. Tahermansouri H, Mirosanloo A, Heidarikeshel S, Gardaneh M. Synthesis characterization and toxicity of multi walled carbon nanotubes functionalized with 4hydroxyquinazoline. Carbon Let2016; 17: 45-52. doi. 10.5714/CL.2016.17.1.045
28. Yang K, Wan J, Zhang S, Tian B, Zhang Y, Liu Z. The influence of surface chemistry and size of nanoscale graphene oxide on photothermal therapy of cancer using ultra low laser power. Biomaterials 2012; 33: 2206-14. doi.10.1016/j.biomaterials.2011.11.064
29. Samuel M S, Selvarajan E, Subramaniam K, Mathimani T, Seethappan S, Pugazhendhi A. Synthesized β-cyclodextrin modified graphene oxide composite for adsorption of cadmium and their toxicity profile in cervical cancer cell
30. lines. Proce Biochem 2020; 93: 28-35. doi. 10.1016/j.procbio.2020.02.014
31. Kheiltash F, Parivar K, Hayati Roodbari N, Sadeghi B, Badiei A. [Effects of 8hydroxyquinoline coated graphene oxide on cell death and apoptosis in MCF-7 and MCF-10 breast cell lines]. Iran J Bas Med Sci 2020; 23:871-8. (Persian)

XML   Persian Abstract   Print

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 29, Issue 5 (11-2021) Back to browse issues page