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Journal of Ilam University of Medical Sciences 2021, 29(6): 90-101 Back to browse issues page
Effect of High Fat Diet and Endurance Training on the Gene Expression of Sarco/Endoplasmic Reticulum ATPase2 (SERCA2) and Ryanodine Receptor2 (RYR2) under Near-Thermoneutrality in Inguinal Adipose Tissue of Mice
Saeed Daneshyar * 1, Amir Khosravi2, Yazdan Fourotan3
1- Dept of Physical Education, Faculty of Humanities, Ayatollah Alozma Boroujerdi University, Lorestan, Iran , s.daneshyar@yahoo.com
2- Dept of Physical Education, Faculty of Humanities, Ayatollah Alozma Boroujerdi University, Lorestan, Iran
3- Dept of Physical Education, Islamic Azad University, Asad-Abad Branch, Hamadan, Iran
Abstract:   (578 Views)
Introduction: Non shivering thermogenesis is partly yielded by a futile-calcium-cycle mechanism that is regulated by Sarco/Endoplasmic Reticulum ATPase2 (SERCA2) and Ryanodine Receptor2 (RYR2).  This study aimed to investigate the effect of a high-fat diet and endurance training under near-thermoneutrality (in which humans live) on the gene expression of SERCA2 and RYR2 in inguinal subcutaneous adipose tissue in mice.
Material & Methods: A total of 28 C57BL/6 male mice were assigned into four groups of seven animals per group. The groups included 1) control 2), high-fat diet (HFD), 3) endurance training (ET), and 4) HFD-ET. The environment temperature was set out at 26°C. The mice in the HFD group were fed a high-fat diet (fat=42%) for 12 weeks. The mice in the ET group underwent ET on the treadmill for six weeks. The mice of the HFD-ET had both the HFD and ET. The real-time-PCR method was used to measure the gene expression of SERCA2a, SERCA2b, and RYR2 in adipose tissue.
(Ethic code: ABRU.AC.IR/15664-96.44)
Findings: Two-way ANOVA showed that the relative gene expression of SERCA2a, SERCA2b, and RYR2 were not significantly affected by HFD, ET, and HFD-ET (P>0.05).
Discussion & Conclusion: The HFD and ET did not change the regulatory proteins of the futile-calcium-cycle under near-thermoneutrality in the beige adipose tissue. Therefore, it was speculated that the non-shivering thermogenesis that is induced by these conditions might occur independently of futile-cycle-calcium.
Keywords: Beige adipose tissue, Futile cycle, Obesity, Thermogenesis
Full-Text [PDF 643 kb]   (178 Downloads)    
Type of Study: Research | Subject: susteenance
Received: 2020/11/11 | Accepted: 2021/07/5 | Published: 2022/02/4
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Daneshyar S, Khosravi A, Fourotan Y. Effect of High Fat Diet and Endurance Training on the Gene Expression of Sarco/Endoplasmic Reticulum ATPase2 (SERCA2) and Ryanodine Receptor2 (RYR2) under Near-Thermoneutrality in Inguinal Adipose Tissue of Mice. sjimu 2021; 29 (6) :90-101
URL: http://sjimu.medilam.ac.ir/article-1-6846-en.html


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مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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