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:: Volume 28, Issue 4 (10-2020) ::
Journal of Ilam University of Medical Sciences 2020, 28(4): 12-24 Back to browse issues page
L-Glutamin-Induced Inhibition of Endotoxemia by Reducing Pre-Inflammatory Cytokines, Nitric Oxide, and CD40 Expression
Yaser Jafari khataylou * 1, Somayeh Ahmadiafshar2
1- Dept of Pathobiology, Faculty of Veterinary medicine, University of Tabriz, Tabriz, Iran , y.jafari@tabrizu.ac.ir
2- Dept of Microbiology, Faculty of Veterinary medicine, University of Urmia, Urmia, Iran
Abstract:   (1956 Views)
Introduction: Researchers have been investigating the major pathogens involved in septic shock caused by lipopolysaccharide in recent years. Moreover, they have been concerned about the use of appropriate drugs to intervene with the main pathogenesis of the disease. This study aimed to investigate the effect of L-Glutamine as an HSP70 inducer in reducing pre-inflammatory cytokines and effective intermediaries in septic shock, including nitric oxide production and CD40 expression in mice treated with lipopolysaccharide (LPS).
Materials & Methods: In total, 25 Swiss Albino mice were randomly divided into three groups of A (control group, n=5), (received LPS with a dose of 0.75 mg/animal intraperitoneally [IP], (n=10), and (received L-glutamine with a dose of 50 mg/kg IP 30 minutes after the injection of LPS [three doses every 24 h], (n=10). Subsequently, one day after the final dose of drug induction, the number of cytokines, such as IFN-γ, IL-4, IL-10, HSP70, CD40, and nitric oxide, were evaluated in this study. Ethics code: IR.TABRIZU.REC.1398.003
Findings: The data were analyzed in SPSS software (version 19) through a one-way analysis of variance and Scheffe test. According to the results, L-glutamine can significantly reduce the serum levels of pre-inflammatory cytokine, nitric oxide, and CD40 expression in the drug-treated group (group C), compared to the LPS- treated group (group B) (P<0.05).
Discussions & Conclusions: L-glutamine could be a good choice for treatment by reducing the level of inflammatory mediators.
Keywords: Heat shock protein70, L-glutamine, Lipopolysaccharide, Nitric oxide
Full-Text [PDF 1058 kb]   (809 Downloads)    
Type of Study: Research | Subject: immunology
Received: 2019/12/18 | Accepted: 2020/05/26 | Published: 2020/10/31
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Ethics code: IR.TABRIZU.REC.1398.003

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Jafari khataylou Y, Ahmadiafshar S. L-Glutamin-Induced Inhibition of Endotoxemia by Reducing Pre-Inflammatory Cytokines, Nitric Oxide, and CD40 Expression. Journal title 2020; 28 (4) :12-24
URL: http://sjimu.medilam.ac.ir/article-1-6227-en.html

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Volume 28, Issue 4 (10-2020) Back to browse issues page
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