:: Volume 28, Issue 6 (1-2021) ::
Journal of Ilam University of Medical Sciences 2021, 28(6): 23-32 Back to browse issues page
Effect of Aerobic Exercise on the Renal Apelin and Nitric Oxide Levels in Male Rats Exposed to Nano Zinc Oxide
Zahra Salleh Ahangar1 , Masoumeh Habibian * 2
1- Dept of Physical Education and Sports Sciences, Sari Branch, Islamic Azad University, Sari, Iran
2- Dept of Physical Education and Sports Sciences, Qaemshahar Branch, Islamic Azad University, Qaemshahar, Iran , habibian_m@yahoo.com
Abstract:   (1865 Views)
Introduction: Nanoparticles accumulate easily in the organs of the body due to their small size. Therefore, an increase in the use of nanoparticles leads to more concerns about their side effects. Accordingly, the present study was conducted to investigate the effect of aerobic exercise on the renal Apelin and Nitric Oxide (NO) levels in male rats exposed to zinc oxide nanoparticles.
 
Materials & Methods: In this experimental study, 35 male Wistar rats with a weight range of 150-170 g were randomly divided into five groups of seven animals per group. The groups included control, salin, zinc oxide nano, exercise, and exercise+zinc oxide nano. The zinc oxide nano (1mg/kg) was administered intraperitoneally 5 times/week to the exercise and exercise+zinc oxide nano groups. The exercise program consisted of progressive running on a treadmill (speed from 15 to 18 m/min, 25 to 44 min/day, five times per week for four weeks). The rats were euthanized two days after the last intervention. The kidney tissues were removed and homogenized to measure the levels of Apelin, NO, and Malondialdehyde (MDA). The data were analyzed through a one-way ANOVA test (P<0.05).
Ethics code: 20821404942014/6-1395
 
Findings: The results indicated that the zinc oxide nano administration significantly increased renal MDA as well as NO and significantly decreased Apelin, compared to the control group. Moreover, the 4-week exercise training was associated with a significant decrease in the renal MDA in exercise and exercise+zinc oxide nano groups and a significant increase in Apelin levels in the exercise group. Exercise also induced a significant increase in the tissue NO levels in the healthy rats; however, it reduced NO level in the kidney of rats exposed to zinc oxide nano.
 
Discussions & Conclusions: It seems that regular exercise can exert its protective role against zinc oxide nanoparticle-induced renal injury by reducing oxidative stress, increasing Apelin, and modifying NO levels in the kidney tissue.
 
Keywords: Apelin, Aerobic exercise, Kidney, Malondialdehyde, Nitric oxide, Nano zinc oxide
Full-Text [PDF 857 kb]   (817 Downloads)    
Type of Study: Research | Subject: education vartqa slamt
Received: 2019/10/1 | Accepted: 2020/08/9 | Published: 2021/02/28
References
1. Xiao L, Liu C, Chen X, Yang. Zinc oxide nanoparticles induce renal toxicity through reactive oxygen species. Food Chem Toxicol 2016; 90:76-83. doi. 10.1016/j.fct.2016.02.002.
2. Scherzad A, Meyer T, Kleinsasser N, Hackenberg S. Molecular mechanisms of zinc oxide nanoparticle induced genotoxicity short running title genotoxicity of ZnO NPs. Materials 2017; 10: 1427. doi.10.3390/ma10121427.
3. Rim KT, Song SW, Kim HY. Oxidative DNA damage from nanoparticle exposure and its application to worker's health a literature review. Saf Health Work 2013; 4:177-86. doi.10.1016/j.shaw.2013.07.006.
4. Hosseini M, Amani R, Razavimehr V, Moshrefi AH, Aghajanikhah MH. [Histopathologic and biochemical study of zinc oxide nanoparticles effect on renal tissue in Rats]. SJIMU 2018; 26:177-86. (Persian) doi.10.29252/sjimu.26.3.177
5. Khorsandi L, Heidarimoghadam A, Jozi Z. Nephrotoxic effects of low dose zinc oxide nanoparticles in Rats. J Nephropathol 2018; 7:158-65. doi. 10.15171/jnp.2018.35
6. Nishida M, Hamaoka K. The apelin APJ system its role in renal physiology and potential therapeutic applications for renal disease. Nephrology 2013; 1:7. doi. 10.13172/2053-0293-1-1-526
7. Nishida M, Okumura Y, Oka T, Toiyama K, Ozawa S, Itoi T, et al. The role of apelin on the alleviative effect of Angiotensin receptor blocker in unilateral ureteral obstruction-induced renal fibrosis. Nephron Ext2012; 2:39-47. doi. 10.1159/000337091
8. Navar LG, Kobori H, Prieto MC. Intrarenal angiotensin II and hypertension. Curr Hypertens Rep 2003; 5:135-43. doi. 10.1007/s11906-003-0070-5
9. Kadkhodaeielyaderani M, Malekaskar AM, Rostami M, Aberomand M, Khirollah A. [Kinetic activity of isolated nitric oxide synthase enzyme from sheep kidney]. J Mazandaran Uni Med Sci 2013; 22:59-69. (Persian)
10. Farahati S, Atarzadehhosseini SR, Bijeh N, Mahjoob O. [The effect of aerobic exercising on plasma nitric oxide level and vessel endothelium function in postmenopausal women]. RJMS2014; 20:78-88. (Persian)
11. Podhorska M, Dziegiel P, Murawska E, Saczko J, Kulbacka J, Gomulkiewicz A, et al. Effects of adaptive exercise on apoptosis in cells of Rat renal tubuli. Eur J Appl Physiol 2007; 99:217-26. doi.10.1007/s00421-006-0335-1
12. Pujalte I, Passagne I, Daculsi R, Portal C, Ohayon Courtes C, Lazou B. Cytotoxic effects and cellular oxidative mechanisms of metallic nanoparticles on renal tubular cells. Toxicol Im Part Sol Res 2015;4: 409-422. (Persian) doi.10.1039/C4TX00184B
13. Fatahi B, Habibian M. [Effect of aerobic exercise on renal angiotensin ii and angiotensin type 1 receptor levels in administered rats with nano zinc oxide]. MJMS2018; 21:29-34. (Persian)
14. Hosseini M, Amani R, Razavimehr V, Moshrefi AH, Aghajanikhah MH. [Histopathologic and biochemical study of zinc oxide nanoparticles effect on renal tissue in rats]. SJIMU2018; 26:177-186. (Persian) doi.10.29252/sjimu.26.3.177
15. Manke A, Wang L, Rojanasakul Y. Mechanisms of nanoparticle-induced oxidative stress and toxicity. Biomed Res Int 2013; 2013:1-15. doi. 10.1155/2013/942916
16. Lee J, Bae EH, Ma SK, Kim SW. Altered nitric oxide system in cardiovascular and renal diseases. Chonnam Med J 2016; 52:81-90. doi.10.4068/cmj.2016.52.2.81.
17. Poontawee W, Natakankitkul O. Protective effect of cleistocalyx nervosum Var paniala fruit extract against oxidative renal damage caused by cadmium. Molecules2016 22; 21:133. doi.10.3390/molecules21020133
18. Ocak S, Gorur S, Hakverdi S, Celik S, Erdogan S. Protective effects of caffeic acid phenethyl ester, vitamin c, vitamin e and-acetylcysteine on vancomycin-induced nephrotoxicity in Rats. Bas Clin Pharmacol Toxicol2007; 100:328-33. doi. 10.1111/j.1742-7843.2007. 00051.x
19. Fadda LM, Abdelbaky NA, Alrasheed NM, Alrasheed NM, Fatani AJ, Atteya M. Role of quercetin and arginine in ameliorating nano zinc oxide induced nephrotoxicity in Rats. BMC Complement Altern Med 2012; 12: 60. doi. 10.1186/1472-6882-12-60
20. Peeri M, Habibian M, Azarbayjani MA, Hedayati M. Protective effect of aerobic exercise against L-NAME induced kidney damage in Rats. Arh Hig Rada Toksikol 2013; 64:43-9. doi. 10.2478/10004-1254-64-2013-2260.
21. Ito D, Ito O, Cao P, Mori N, Suda C, Muroya Y, et al. Effects of exercise training on nitric oxide synthase in the kidney of spontaneously hypertensive rats. Clin Exp Pharmacol Physiol2013; 40:74-82. doi.10.1111/1440-1681.12040
22. Mahmoody AA, Dabidiroshan V, Gharakhanlou R, Hedayati M. [Effects of exercise and ferula gummosa on apelin of cardiac and kidney tissues in L-NAME induced hypertension in Rats]. Iranian J Health Phys Act 2013; 4:42-50. (Persian)
23. Zhang J, Ren CX, Qi YF, Lou LX, Chen L, Zhang LK, et al. Exercise training promotes expression of Apelin and APJ of cardiovascular tissues in spontaneously hypertensive rats. Life Sci 2006 15; 79:1153-9. doi.10.1016/j.lfs.2006.03.040
24. Chen H, Wan D, Wang L, Peng A, Xiao H, Petersen RB, et al. Apelin protects against acute renal injury by inhibiting TGF-β1. Biochim Biophys Acta2015; 1852:1278-87. doi.10.1016/j.bbadis.2015.02.013.
25. Lv D, Li H, Chen L. Apelin and APJ a novel critical factor and therapeutic target for atherosclerosis. Acta Biochim Biophys Sin Shanghai 2013;45: 527-33. doi. 10.1093/abbs/gmt040

Ethics code: 20821404942014/6-1395

XML   Persian Abstract   Print

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 6 (1-2021) Back to browse issues page