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Evaluation of Antioxidant and Antibacterial Activity, and Determination of Phenolic and Flavonoid
Content of Aqueous and Methanolic
Extracts of Scutellaria
pekinensis
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Mostafa Govahi *    1, Fatemeh Ghorbani2 , Mojtaba Ranjbar3 , Somayeh Rahaiee3 , Hossein Azizi4 |
1- Dept of Nanobiotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran , m.govahi@ausmt.ac.ir
2- Dept of Medicinal Plants, Faculty of Medicinal Plants, Amol University of Special Modern Technologies, Amol, Iran
3- Dept of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran
4- Dept of Nanobiotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran |
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Abstract: (4000 Views) |
Introduction: Scutellaria pekinensis is one of the most valuable Medicinal species of the Lamiaceae family. The present study aimed to investigate the antibacterial and the antioxidant effects of Scutellaria pekinensis.
Materials & Methods: In this study, after extracting the aqueous and methanol extracts, the antioxidant effect of the extract was measure-using DPPH and FRAP methods. Disk diffusion method was use to investigate the antibacterial effect against three types of bacteria.In addition, the study examined phenolic and flavonoidal.
Findings: The results showed that the highest amount of phenol and flavonoid were obtained by methanol extract and shaker extraction method. The highest amount of free radical scavenging (DPPH) and FRAP were related to aqueous extract and shaker extraction method. The highest inhibition zone diameter for Bacillus cereus and Escherichia coli in the concentration of 600 mg/ml were 15.67±0.58, and 25.33±3.15 respectively, and for Staphylococcus aureus, the concentration of 900 mg / ml was 26±2.
Discussion & Conclusions: The results showed that the solvent type and extraction method had a great impact on the amount of antioxidant compounds and antibacterial effects. Considering the few studies performed about this plant, the results of this study can be a good report for further research.
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| Keywords: Antioxidant, Antibacterial, DPPH, Folin Reagent |
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Full-Text [PDF 757 kb]
(1324 Downloads)
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Type of Study: Research |
Subject:
Bacteriology
Received: 2019/02/4 | Accepted: 2019/06/18 | Published: 2019/09/15
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