:: Volume 27, Issue 3 (9-2019) ::
Journal of Ilam University of Medical Sciences 2019, 27(3): 183-193 Back to browse issues page
The Study of the Effects of Acetobacter Isolated from Local Picklesin PTEN/AKT Signaling Route in Colon Cancer Cells (HT29)
Pariya Azarng1 , Changiz Ahmadizadh * 2
1- Dept of Biology, Bonab Branch, Islamic Azad University, Bonab,Iran
2- Dept of Microbiology, Ahar Branch , Islamic Azad University, Ahar,Iran , dr_ahmadizadeh@yahoo.com
Abstract:   (2518 Views)
Introduction: The imbalance of the microbial ecosystem causes biological changes associated with colorectal cancer and cell proliferation and their planned death, as well as the immune responses. The aim of this study was to determine the effect of probiotic bacteria on the expression of genes associated with growth, metastasis and apoptosis in cancer cells of the HT29 colon.
Materials & Methods: During this study, probiotic Acetobacter were cultured and treated with HT29 cancer cells after preparation of media. The MTTassay method was investigated. DNA extraction from the treated cells was performed and the DNA Ladder assay test was performed. After extraction of RNA and preparation of cDNA, the expression of genes related to growth (AKT1 and PTEN) and apoptosis pathway gene (BAX) was measured by Real-timePCR.
Findings: The results of the MTT assay showed that the Acetobacter bacteria inhibited the proliferation of HT-29 cells and induced apoptosis in these cells. The DNA ladder assay results from treatment of HT29 cells with these bacteria showed qualitative changes in cell apoptosis. In addition, the results of Real-time PCR indicated that the bacterial strains increased the expression of BAX (PTEN) genes in the cancer cells of the HT29 colon.

Discussion & Conclusions: Acetobacter stimulates cellular apoptosis signaling pathway in cancer cells of the HT29 colon and it can be used as an adjuvant therapy in colon cancer treatment or in new treatment strategies.
Keywords: Colorectal cancer, Acetobacter, HT29
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Type of Study: Research | Subject: Microbiology
Received: 2018/07/14 | Accepted: 2019/01/20 | Published: 2019/09/15

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Volume 27, Issue 3 (9-2019) Back to browse issues page