:: Volume 28, Issue 5 (11-2020) ::
Journal of Ilam University of Medical Sciences 2020, 28(5): 33-42 Back to browse issues page
Protective Effects of Astaxanthin on the Levels of Urea and Creatinine as well as Changes in Kidney Tissue following Cadmium-Induced Toxicity in Syrian Male Mice
Nazanin Fathi1 , Fereshteh Mir Mohammadrezaei * 2, Akbar Hajizadeh moghadam1
1- Dept of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
2- Dept of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran , fereshteh.mmrezaei@gmail.com
Abstract:   (2423 Views)
Introduction: Cadmium is an environmental and industrial pollutant that causes nephrotoxicity. Astaxanthin is a carotenoid with antioxidant, anti-apoptotic, and anti-inflammatory properties. This study investigated the protective effect of astaxanthin on the levels of urea and creatinine, as well as kidney tissue in cadmium-induced nephrotoxicity.
 
Materials & Methods: In total, 42 mice were divided into seven groups of control, sham (received saline and olive oil), cadmium (received cadmium at a dose of 1 mg/kg for 14 days [sub-acute] and a dose of 3 mg/kg for 1 day [acute]), and treatments (received a dose of 10 mg/kg astaxanthin for 14 days with 1 mg/kg cadmium along with astaxanthin for 14 days and 3 mg/kg cadmium at the last day). It is worth mentioning that the injection was intraperitoneally and each group included six animals. The levels of creatinine and urea were evaluated in the serum, and the kidney sections were stained with hematoxylin-eosin. Ethics code: IR.UMZ.REC.1397.107
Findings: The results showed a significant increase in the level of urea at an acute dose of cadmium, compared to the sham group (P<0.05). However, urea level had no changes at the sub-acute dose of cadmium. Moreover, Astaxanthin significantly decreased the level of urea at the acute dose of cadmium (P<0.001), compared to the level of urea at the sub-acute dose. Furthermore, cadmium and astaxanthin did not alter the creatinine level. Cadmium causes dilation and bleeding of the renal tubular, compared to the control group; however, astaxanthin improved tissue injury induced by cadmium.
 
Discussions & Conclusions: Astaxanthin with the protective effect on kidney tissue and the improvement of glomerular filtration prevents nephrotoxicity induced by cadmium.
 
Keywords: Astaxanthin, Cadmium, Kidney tissue, Mice, Urea
Full-Text [PDF 964 kb]   (702 Downloads)    
Type of Study: Research | Subject: animal physiology
Received: 2018/01/13 | Accepted: 2018/08/6 | Published: 2020/12/30
References
1. Park YM, Kwan MP. Individual exposure estimates may be erroneous when spatiotemporal variability of air pollution and human mobility are ignored. Health Place 2017; 43:85-94. doi.10.1016/j.healthplace.2016.10.002.
2. Micali A, Pallio G, Irrera N, Marini H, Trichilo V, Puzzolo D, et al. Flavocoxid a protects mouse kidney from cadmium natural antioxidant induced toxicity. Oxid Med Cell Lon 2018;2018. doi.10.1155/2018/9162946.
3. Ansari MA, Raish M, Ahmad A, Alkharfy KM, Ahmad SF, Attia SM, et al. Sinapic acid ameliorate cadmium induced nephrotoxicity in vivo possible involvement of oxidative stress apoptosis and inflammation via NF-κB downregulation. Environ Toxicol Pharmacol 2017; 51: 100-7. doi.10.1016/j.etap.2017 .02.014.
4. Huang M, Su L, Yang L, Zhu L, Liu Z, Duan R. Effect of exogenous TGF-β1 on the cadmium-induced nephrotoxicity by inhibiting apoptosis of proximal tubular cells through PI3K-AKT-mTOR signaling pathway. Chem Biol Interact 2017; 269:25-32. doi.10.1016/j.cbi.2017.03.010.
5. Eom SY, Seo MN, Lee YS, Park KS, Hong YS, Sohn SJ, Kim YD, et al. Low-level environmental cadmium exposure induces kidney tubule damage in the general population of Korean adults. Arch Environ Contam Toxicol2017;73: 401-9.doi. 10.1007/s00244-017-0443-4.
6. Elkhadragy MF, Alolayan EM, Alamiery AA, Moneim AE. Protective effects of Fragaria ananassa extract against cadmium chloride induced acute renal toxicity in Rats. Bio Trace Elem Res 2018; 181:378-87. doi.10.1007/s12011-017-1062-7.
7. Hosten AO. Clinical methods the history physical and laboratory examinations. 3th ed. Sunders Publication. 1990; P.874-8.
8. Bostom AG, Kronenberg F, Ritz E. Predictive performance of renal function equations for patients with chronic kidney disease and normal serum creatinine levels. J Am Soc Neph 2002; 3:2140-4. doi.10.1097/01.asn.0000022011. 35035.f3.
9. Das K, Roychoudhury A. Reactive oxygen species and response of antioxidants as ROS-scavengers during environmental stress in plants. Front Environ Sci 2014;2:53. doi. 10.3389/fenvs.2014.00053
10. Jan AT, Azam M, Siddiqui K, Ali A, Choi I, Haq QM. Heavy metals and human health mechanistic insight into toxicity and counter defense system of antioxidants. Int J Mol Sci 2015; 16:29592-630. doi.10.3390/ijms161226183
11. Fassett RG, Coombes JS. Astaxanthin, oxidative stress inflammation and cardiovascular disease. Future Cardiol 2009; 5:333-42. doi. 10.2217/fca.09.19.
12. Li J, Dai W, Xia Y, Chen K, Li S, Liu T, et al. Astaxanthin inhibits proliferation and induces apoptosis of human hepatocellular carcinoma cells via Inhibition of NF-κB P65 and Wnt/β-catenin in vitro. Mar Drug 2015; 13:6064-81. doi. 10.3390/md13106064
13. Kim JH, Nam SW, Kim BW, Kim WJ, Choi YH. Astaxanthin improves the proliferative capacity as well as the osteogenic and adipogenic differentiation potential in neural stem cells. Food Cheml Toxicol 2010; 48:1741-5. doi.10.1016/j.fct.2010.04.002.
14. Wu H, Niu H, Shao A, Wu C, Dixon BJ, Zhang J, et al. Astaxanthin as a potential neuroprotective agent for neurological diseases. Mar Drug2015; 13:5750-66. doi. 10.3390/md13095750
15. Ghlissi Z, Hakim A, Sila A, Mnif H, Zeghal K, Rebai T, et al. Evaluation of efficacy of natural astaxanthin and vitamin E in prevention of colistin-induced nephrotoxicity in the rat model. Environm Toxicol Pharmacol 2014; 37:960-6. doi. 10.1016/j.etap.2014.03.004.
16. Oliveira H, Spano M, Santos C, Lourdes M. Adverse effects of cadmium exposure on mouse sperm. Reproduc Toxicol 2009; 28:550-5. doi. 10.1016/j.reprotox.2009.08.001.
17. Bu T, Mi Y, Zeng W, Zhang C. Protective effect of quercetin on cadmium‐induced oxidative toxicity on germ cells in male Mice. Anat Rec 2011; 294:520-6. doi. 10.1002/ar.21317.
18. Wang X, Zhao H, Shao Y, Wang P, Wei Y, Zhang W, et al. Nephroprotective effect of astaxanthin against trivalent inorganic arsenic-induced renal injury in wistar Rats. Nutr Res pract 2014; 8:46-53. doi.10.4162/nrp.2014.8.1.46
19. Manna P, Sinha M, Sil PC. Taurine plays a beneficial role against cadmium induced oxidative renal dysfunction. Am Ac 2009; 36:417. doi.10.1007/s00726-008-0094-x.
20. Shagirtha K, Pari L. Hesperetin a citrus flavonone attenuates cadmium induced nephrotoxicity in Rat. Biomed Preve Nutr 2011; 1:139-45. doi. 10.1016/j.ecoenv.2011.06.002
21. Gabr SA, Alghadir AH, Ghoniem GA. Biological activities of ginger against cadmium induced renal toxicity. Saudi J Biolog Sci 2017; 18. doi.10.1016/j.sjbs.2017.08.008.
22. Augusti PR, Conterato GM, Somacal S, Sobieski R, Spohr PR, Torres JV, et al. Effect of astaxanthin on kidney function impairment and oxidative stress induced by Mercuric chloride in Rats. Food Chem Toxicol 2008; 46:212-9. doi.10.1016/j.fct.2007.08.001.
23. Renugadevi J, Prabu SM. Cadmium induced hepatotoxicity in Rats and the protective effect of naringenin. Exp Toxicol Path 2010; 62:171-81. doi. 10.1016/j.etp.2009.03.010.

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