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Evaluation of the Effects of Co-administration of Humulus lupulus hydroalcoholic Extract with Ketamine on Anesthetic Parameters in Male Rat
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Mohammad Yarmohammadpoor-Beneh- Khalkhal1   , Saeid Abbasi Maleki * 2, Azam Bakhtiarian3 |
1- Dept of Pharmacology, Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran
2- Dept of Pharmacology and Toxicology, Urmia Branch, Islamic Azad University, Urmia, Iran , s.abbasi@iaurmia.ac.ir
3- Dept of Pharmacology, Tehran University of Medical Science, Tehran, Iran |
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Abstract: (4419 Views) |
Introduction: Previous studies have shown sedative, hypnotic, anti-anxiety, and pre-anesthetic properties of Humulus lupulus L. (HL). In the present study, the effect of anesthesia with a combination of HL with ketamine on anesthetic parameters among male rats was evaluated.
Materials & methods: 32 male rats were randomly divided into 4 groups of eight in each and then animals intraperitoneally received Humulus lupulus(HL) (100mg/kg), Ketamine(K) (50 mg/kg), Humulus lupulus with ketamine (HLK) (100mg/kg and 50mg/kg, respectively) and diazepam with ketamine(DK) (2.5 and 50 mg/kg, respectively). Induction time, surgical anesthesia(SA) time, walking time, heart and respiratory rate, temperature, and withdrawal reflexes (lip, tail and pedal pinches) were recorded.
Findings: HL group could not induce SA. However, the induction time, SA, walking time, and even withdrawal reflexes of HLK group were similar to the DK group (p>0.05). K group had late and short anesthesia compared to the other two groups (p<0.05).The heart rate and the respiratory rate of HLK group increased compared to DK group (p<0.05). The body temperature of HLK group was higher than DK group (p<0.05).
Discussion & conclusions: Anesthesia of HLK group was similar to DK group and probably components of the HL extract with GABAergic activity responsible for their pre-anesthetic activity. However, further studies are required to clarify the exact mechanisms of action.
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| Keywords: Humulus lupulus, Hydroalcoholic extract, Ketamine, GABAergic System, Anesthesia, Rat |
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Full-Text [PDF 647 kb]
(1603 Downloads)
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Type of Study: Research |
Received: 2017/05/28 | Accepted: 2017/09/13 | Published: 2018/01/16
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