:: Volume 26, Issue 5 (1-2019) ::
Journal of Ilam University of Medical Sciences 2019, 26(5): 51-60 Back to browse issues page
Effects of Pathogenesis Outcomes on Adherence Rate and Gene Toxicity in Helicobacter pylori
Zeynab Fazeli Bavandpoor1 , Masoud Albouyeh * 2, Mostafa Rezaie Tavirani3 , Mohammad Reza Zali3
1- Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid , Masoud.Alebouyeh@gmail.com
3- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract:   (4267 Views)

Introduction: The clinical outcome of Helicobacter pylori in the infected patients depends on the genetic diversity of H. pylori strains. Colonization and adherence on cell host is the first step of the bacteria pathogenesis. This study was aimed to understand the role of pathogenesis factors of H. pylori strains and adherence rate of these strains on AGS cell line, as well as exploring the association between these rates with bacteria pathogenesis outcomes.
Materials & Methods: This study was conducted on genes, including iceA, cagA, babA, vacA, and cag-PAI in clinical strains of H. pylori with different pathogenesis outcomes through polymerase chain reaction (PCR) analysis using specific primers. Moreover, the adherence assay was performed on AGS for exploring the association between adherence rate and pathogenesis outcomes in infected patients.
Findings: The analysis of PCR results indicated that in strains with severe diseases outcomes all the investigated genes were present, while in strain with moderate outcome only adherence genes were present. Furthermore, the results of adherence analysis were indicative of the difference among these strains. Accordingly, two strains with severe pathogens outcomes had the highest rate of colonization. Minimum colonization rate and moderate pathogenesis outcome were observed in other strains, although adherence genes present.
Discussion & Conclusions: According to the results of the current study, it can be concluded that different genes are contributing to bacterial adherence on host cells, meaning that the presence or absence of one or two of these genes may not affect its adherence to host cells. Furthermore, adherence genes along with oncogens cause severe clinical outcomes in infected patients.

Keywords: Helicobacter pylori, Adherence rate, Pathogenesis
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Type of Study: Research | Subject: Medical microbiology
Received: 2017/02/6 | Accepted: 2017/08/14 | Published: 2019/01/15

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Volume 26, Issue 5 (1-2019) Back to browse issues page