:: Volume 25, Issue 5 (1-2018) ::
Journal of Ilam University of Medical Sciences 2018, 25(5): 133-145 Back to browse issues page
Evaluating the Effects of Carnosic acid on TNF-α Gene Expression in LPS-activated Synoviocytes
Mehranoush Saffarpour1 , Hossein Maghsoudi * 2, Mohammad Fazilati3
1- Education and Training
2- Payamenoor university , hosseinm2002@gmail.com
3- Paymnoor university
Abstract:   (4218 Views)

Introduction: Osteoarthritis (OA) is a major cause of disability among adults. As a progressive degenerative joint disorder, OA is characterized by cartilage damage, changes in the subchondral bone and inflammation of the synovium tissue and tendon. Non-pharmacologic agents modulating pro-inflammatory mediator expression offer considerable promise as safe and effective treatments for OA. The aim of this study was to evaluate the effects of Carnosic acid (CA) on TNF-α gene expression in synoviocytes.
Material & methods: To determine the dose response of CA, synoviocytes (5 × 105 cells/well) were incubated at 5% CO2, 37ºC for 72 h with (1) control media alone or (2) CA at concentrations of 0.01, 0.09, 0.1, 0.9, 1, 9,10and 90 mg/ml. Synoviocytes were treated with LPS (20 ng/ml) for 1h and TNF-α mRNA were measured by both reverse-transcriptase PCR and Real-time PCR.
Findings: CA reduced TNF-a, expression in LPS-activated synoviocytes to levels similar to nonactivated control levels. Cells activated with 20 ng/ml LPS showed a significant upregulation of TNF-α expression. In activated synoviocytes cells pretreated with CA, TNF-α was reduced by 48/11% when compared to activated control cells.
Discussion & conclusions: This study demonstrates that CA acts as a potent inhibitor of TNF-α. Previous research has shown that these mediators are important in the pathogenesis of OA and reduction in these mediators has been associated with amelioration of cartilage breakdown.

Keywords: Osteoarthritis, Carnosic acid, TNF-α, synoviocyte
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Type of Study: Research |
Received: 2016/06/20 | Accepted: 2016/08/13 | Published: 2018/01/15

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Volume 25, Issue 5 (1-2018) Back to browse issues page