1- Dept of Microbiology, Faculty of Sience,Sanandaj Branch, Islamic Azad University, Sanandaj, Iran , sheidazonoori@gmail.com 2- Dept of Microbiology, Faculty of Sience,Sanandaj Branch, Islamic Azad University, Sanandaj, Iran 3- Dept of Biochemistry Facultyof Sience, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
Abstract: (5803 Views)
Introduction: Today, emergence of extended spectrum betalactamase(ESBL) producing organisms are one of the health problems. Extended spectrum betalactamases are enzymes which cause the resistance to betalactam antibiotics. The aim of this study was to determine the antibiotic resistance pattern and prevalence of SHV and SHV-1 genes in ESBL- producing Escherichia coli isolated from urinary tract infection in Sanandaj.
Materials & methods: 150 isolated Escherichia coli from 406 urinary samples from patients referring to health center of Sanandaj were collected. All Eschrichia coli samples were identified by conventional biochemical tests. Antibiotic susceptibility testing was performed by disk diffusion method. Combined disk was also utilized as a confirmatory test, and results were compared with CLSI standards. The ESBL positive isolates were investigated by PCR for detecting SHV and SHV-1 genes.
Findings: From total of 150 Eschrichia coli isolates, resistance to ceftriaxone, pipracillin, gentamycin, amikacin, imipenem, ciprofloxacin, carbenicillin, cefepime, ceftazidime and cefotaxime were 29%, 58.5%, 4.66%, 10%, 33.25%, 34.66%, 26%, 22%, 26% and 28.66% respectively. 43 isolates were ESBL positive and among them 34 isolates (79.6%) contained SHV and 32 isolates (74.41%) had SHV-1 gene.
Discussion & conclusions: According to the results, the pravalence of antibiotic resistant strains and also ESBL producing strains in Sanandaj is high and finding ways to prevent the spread of these stains is important.
zonouri S S, Davari K, mirzaii S. The Prevalence of SHV and SHV-1 Type of Extended-Spectrum-Betalactamase Genes in Escherichia coli Strains Isolated from Urine Samples of Patients Referring to Health Centers of Sanandaj. J. Ilam Uni. Med. Sci. 2017; 25 (2) :27-34 URL: http://sjimu.medilam.ac.ir/article-1-3304-en.html