:: Volume 23, Issue 2 (inpress 2015) ::
Journal of Ilam University of Medical Sciences 2015, 23(2): 141-148 Back to browse issues page
Prevalence of metallo-β-lactamase blaIMP and blaVIM genes in urinary isolates of Pseudomonas aeruginosa in Ilam
Mohamad Maspi *1, Fatemeh Ghanbari2, Mojtaba Darboie1, Noorkhoda Sadeghifard2
1- branch, Islamic Azad University Fars
2- Ilam University of Medical Sciences
Abstract:   (8698 Views)

Introduction: class B beta-lactamases such as IMP and VIM that read metallo-β-lactamase(MBL), due to the hydrolysis of penicillins, cephalosporins and carbapenems for the teatment of infectious diseases, major difficulties create. In study, the prevalence of MBL blaIMP and blaVIM genes in urinary isolates of Pseudomonas aeruginosa were investigated in ilam province.
Materials & methods: A total of 60 strains of P. aeruginosa Central Laboratory of ilam, were collected and were identified using biochemical methods Antibiotic susceptibility testing by the disk diffusion method(Kirby-Bauer) forantibiotics cefepiem, ceftriaxone, Aztreonam, gentamicin, amikacin, ciprofloxacin, ofloxacin, ceftazidime, piperacillin tazobactam and imipenem was performed. Then strain insensitive to ceftazidime, the method Ceftazidime - EDTA Combined disk synergy test(CDST-CAZ) Was used to detect MBL-producing strains. The PCR test strains using specific primers for blaIMP and blaVIM genes was investigated.
Findings: A total of 17 strains were resistant to ceftazidime. With method CDST all strains non-susceptible to ceftazidime, were MBL-producers. PCR and sequencing methods proved that 6 isolates were positive for blaVIM genes, whereas none were positive for blaIMP genes. All isolates that were positive for VIM gene were resistant to all antibiotics studied except piperacillin tazobactam.
 Discussion & conclusion :In this study, blaVIM dominant gene in P. aeruginosa urinary strains resistant to ceftazidime was administrative. Given the importance of MBL-producing strains in hospitals, rapid detection of these strains could be crucial step in the treatment and control of infections caused by these strains is considered.

Keywords: MBL, Pseudomonas aeruginosa, Antibiotic resistance
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Type of Study: Research | Subject: Molecular Genetics
Received: 2015/07/20 | Accepted: 2015/07/20 | Published: 2015/07/20

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Volume 23, Issue 2 (inpress 2015) Back to browse issues page