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Investigation on the Inhibitory Effects of Hydro-alcoholic Extract of Thymbra spicata on the Growth of Lung Cancer Cell Line SK-Mes-1
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S Sabz ali   , A Rostam zad , J Panahi , M Havasian , K Haghani , S Bakhtiary *1 |
| 1- , bakhtiyari-s@medilam.ac.ir |
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Abstract: (10069 Views) |
| Introduction: Lung cancer is one of the main
causes of death among cancers worldwide.
Since the most of current treatments for
cancer are inefficient and have several side
effects, finding an effective and alternative
treatment has a great importance. To this
end, medicinal plants have gained a special
significance in recent years. The aim of this
study was to examine the inhibitory effects
of hydro-alcoholic extract of Thymbra spicata
on the growth of lung cancer cell line
SK-Mes-1.
Materials & Methods: After cultivation of
SK-MES-1 cells, these cells were treated
with the several doses of hydro-alcoholic
extract of Thymbra spicata for 24, 48, 72
hours to quantify IC50 value. After treatment,
MTT was performed. Furthermore, to
evaluate DNA fragmentation, DNA was
extracted 24 hours after treatment and
electrophoresis was done on agarose 1%.
Findings: The results of MTT indicated that
this extract has a dose-dependent cytotoxic
effect on SSK-MES-1 cells. IC50 of this
extract was determined as 110 microgram/
ml for SK-MES-1 cells. MTT results at
48 and 72 hours had a no significant difference
with 24 hours. Also, treatment with
this extract had no any effect on DNA fragmentation.
Discussion & Conclusion: Results of this
study indicated that hydro-alcoholic extract
of Thymbra spicata could inhibit the growth
of lung cancer cell SK-MES-1 through direct
cytotoxic effects. Further studies are suggested
to be conducted on cell growth pathways
to determine the molecular mechanisms
of this inhibitory effect. |
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| Keywords: hydro alcoholic extract, Thymbra spicata, SK-Mes-1, lung cancer |
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Full-Text [PDF 518 kb]
(3205 Downloads)
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Type of Study: Research |
Subject:
toxicology
Received: 2014/09/13 | Accepted: 2014/09/13 | Published: 2014/09/13
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