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Survey and Comparison of Immunization Scale of The Recombinant Proteins of Attachment Subunit of Tetanus and Botulinom(A) Toxins
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E Rezaee   , E Miri , J Salimian * 1, Gh Olad , M Sadati , M Ebrahimi , H Bostani |
| 1- , jafarsalimyan@yahoo.com |
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Abstract: (12210 Views) |
| Introduction: The only presented vaccine against tetanospasmin (the main factor of the fatal disease tetanus) is its toxoid which creates a 10-year period immunization. Ho-wever, the botulinum toxoid (the main factor of the fetal disease botulism) only creates a 2-year period immunization. The-se two toxins have 36% homology and are falling into one family but are different in immunological memory, the scale of hom-oral immunity response and probably the scale of antibody production. The goal of this study was to compare the immunization and antibody titer of fragment C of both tetanus toxin (THc) and botulinom type A (BONT/A-Hc).
Materials & Methods: Expression of the recombinant proteins, THc and BONT/A-Hc, was accomplished by using of the tran-sgenic host, E.coli Bl21 DE3 and the pET28a vectors which contained the two genes in an optimum condition. Both the mentioned proteins were derived and puri-fied from the solution phase of cell extract and were evaluated on SDS-PAGE gel. Finally, the antibody titer containing in the serum of immunized animals with each of the aforementioned proteins, was inv-estig-ated and compared by immunoassay test.
Findings: The result of SDS-PAGE gel evaluation showed a proper expression and purification of both the recombinant prot-eins, THc and BONT/A-Hc, in solution phase of cell extract. The immunoassay res-ults of serum showed that the antibody titer against the recombinant protein of THc was more than those for the recombinant protein of BONT/A-Hc.
Discussion & Conclusion: It is conceived that the significant differences in the antib-ody titer can be related to the longevity of memory cells. However, the result needs more studies. |
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| Keywords: antibody titer, immunization, memory cell, tetanus toxin, botulinom tox-in |
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Full-Text [PDF 420 kb]
(2745 Downloads)
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Type of Study: Applicable |
Subject:
Medical physics
Received: 2013/11/11 | Accepted: 2013/11/11 | Published: 2013/11/11
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