[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations62733583
h-index2719
i10-index18478

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 29, Issue 3 (8-2021) ::
Journal of Ilam University of Medical Sciences 2021, 29(3): 26-37 Back to browse issues page
Comparison of Antioxidant, Antibacterial, and Cytotoxic Effects of Essential Oil and Nanoemulsion of Clove Essential Oil
Atena Vafayi Malek1 , Ehsan Karimi * 2, Ehsan Oskoueian3
1- Dept of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2- Dept of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran , ehsankarimi@mshdiau.ac.ir
3- Mashhad Branch, Agricultural Biotechnology Research Institute, Agricultural Research, Education and Extension Organization, Mashhad, Iran
Abstract:   (1801 Views)
Introduction: Medicinal plants are among the most important products in agriculture and medicine. The usefulness and cheapness, environmental friendliness, and limited side effects of medicinal plants have increased the rate of use of these plants in recent years. Plant extracts, such as cloves, have long been used in medicine, perfumery, and as additives in cooking. This study aimed to evaluate and compare the antioxidant, antibacterial, and anti-cancer effects of clove essential oil and nanoemulsion.
 
Materials & Methods: Antioxidant activity of essential oil and clove nanoemulsion by measuring the inhibitory activity of DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS radicals (2 and 2-azino-bis-3-ethyl benzothiazoline-6-sulfonic acid) was measured in this study. The evaluation of antibacterial and bactericidal properties of essential oil and clove nanoemulsion was conducted by culture medium and suspension of four pathogenic bacteria of gram-positive, including Staphylococcus aureus and Bacillus subtilis, and gram-negative bacteria, including Escherichia coli and Pseudomonas aeruginosa, followed by the measurement of the diameter of the growth inhibition zone. In addition, the effects of cytotoxicity of clove essential oil and nanoemulsion on different concentrations of 15.6, 31.2, 62.5, 125, and 250 μg/ml on MCF7 cancer cells were evaluated by MTT staining.
 
Findings: Clove nanoemulsion, compared to essential oil, had good antioxidant potential and a significant effect on free radical scavenging. The rates of free radical scavenging (IC50) of clove essential oil nanoemulsion were obtained at 67.29 and 78.39 μg/ml measured by DPPH and ABTS methods, respectively. Clove essential oil and nanoemulsion had an inhibitory effect on both groups of gram-positive and gram-negative bacteria tested in this study. The toxicity test also showed a greater effect of clove nanoemulsion in killing breast cancer cells, compared to the clove essential oil.
 
Discussions & Conclusions: The results of this in vitro study indicate the antioxidant and anti-cancer effects of clove nanoemulsion, compared to essential oil, which can be used to prevent and treat many diseases associated with the development of reactive oxygen species.
Keywords: Antioxidant, Antibacterial, Anticancer, Nanoemulsion, clove essential oil
Full-Text [PDF 606 kb]   (556 Downloads)    
Type of Study: Research | Subject: biotechnolohgy
Received: 2020/07/21 | Accepted: 2021/06/19 | Published: 2021/09/1
References
1. Elsaber Batiha G, Magdybeshbishy A, Elmmleeh AM. Abdeldaim M, Prasaddevkota H. Traditional uses bioactive chemical constituents and pharmacological and toxicological activities of glycyrrhiza glabra L. Biomolecules 2020;10:352. doi.10.3390/biom10030352
2. Sultana B, Anwar F, Mushtaq M, Aslam M, Ijaz S. Invitro antimutagenic antioxidant activities and total phenolics of clove Syzygium aromaticum L. seed extracts. Pak J Pharm Sci2014;27:53-7.
3. Bhowmik D, Kumar KPS, Yadav A, Srivastava S, Paswan S, Dutta AS. Recent trends in Indian traditional herbs Syzygium aromaticum and its health benefits. J Pharmacogn Phytochem 2012;1:13-22.
4. Alawiyah AL, Senania A, Sari H, Perdana F, Musthafa I. Antioxidant activity of volatile compounds from Syzygium aromaticum L. leaves. J Phys Con Ser2019;2:55038. doi.10.1002/ffj.3299
5. Elmaati MFA, Mahgoub SA, Labib SM, Algaby AMA, Ramadan MF. Phenolic extracts of clove Syzygium aromaticum with novel antioxidant and antibacterial activities. Eur J Integr Med 2016; 4:494-504. doi.10.1016/j.eujim.2016.02.006
6. Aziz ZAA, Ali SAM, Ahmad A, Mohd-Setapar SH. Application of herbal extract and its medicinal value. Der Pharm Lett 2016;8:161-7.
7. Chua LK, Lim CL, Ling APK, Chye SM, Koh RY. Anticancer potential of syzygium speciesa review. Plant Foods Hum Nutr 2019;74:18-27. doi.10.1007/s11130-018-0704-z
8. Wael S, Nuringtyas TR, Wijayanti N, Astuti P. Secondary metabolites production in clove Syzygium aromaticum chemical compounds. J Biol Sci 2018;18:399-406. doi. 10.1016/j.ics.2006.02.002
9. Kaur K, Kaushal S, Rani R. Chemical composition antioxidant and antifungal potential of clove Syzygium aromaticum essential oil its major compound and its derivatives. J Essent Oil Bear Plants 2019;22:1195-217.
10. Severino P, Fangueiro JF, Ferreira S V, Basso R, Chaud M V, Santana MHA, et al. Nanoemulsions and nanoparticles for non-melanoma skin cancer effects of lipid materials. Clin Transl Oncol 2013;15:417-24. doi.10.1007/s12094-012-0982-0
11. Tayeb HH, Sainsbury F. Nanoemulsions in drug delivery formulation to medical application. Nanomedicine2018;19:2507-25. doi.10.2217/nnm-2018-0088
12. Arifin SF, Al Shami A, Omar SSS, Jalil MAA, Khalid KA, Hadi H. Impact of modern technology on the development of natural-based products. J Ayurvedic Herb Med 2019;5:133-42.
13. Aswathanarayan JB, Vittal RR. Nanoemulsions and their potential applications in food industry. Front Sustain Food Syst2019;3:95. doi.10.3389/fsufs.2019.00095
14. Jaiswal M, Dudhe R, Sharma PK. Nanoemulsion an advanced mode of drug delivery system. Biotech 2015;5:123-7. doi.10.1007/s13205-014-0214-0
15. Sahu P, Das D, Mishra VK, Kashaw V, Kashaw SK. Nanoemulsion a novel eon in cancer chemotherapy. Mini Rev Med Chem 2017;17:1778-92. doi. 10.2174/1389557516666160219122755
16. Perez F, Adachi J, Bonomo RA. Antibiotic resistant gram negative bacterial infections in patients with cancer. Clin Infect Dis2014;59: 335-9. doi.10.1093/cid/ciu612
17. Sharifirad J, Sureda A, Tenore G, Daglia M, Sharifrad M, Valussi M, et al. Biological activities of essential oils from plant chemoecology to traditional healing systems. Molecules 2017;22:70.
18. Torre MP, Cavero RY, Calvo MI, Vizmanos JL. A simple and a reliable method to quantify antioxidant activity invivo. Antioxidants 2019;8:142.
19. Shahavi MH, Hosseini M, Jahanshahi M, Meyer RL, Darzi GN. Evaluation of critical parameters for preparation of stable clove oil nanoemulsion. Arab J Chem2019;12:3225-30.
20. Periasamy VS, Athinarayanan J, Alshatwi AA. Anticancer activity of an ultrasonic nanoemulsion formulation of nigella sativa L. essential oil on human breast cancer cells. Ultrason Sonochem 2016;31:449-55.
21. Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant rich US foods. J Food Compos Anal 2011;24:1043-8.
22. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 1995; 28:25-30.
23. Oyaizu M. Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J Nutr Diet 1986;44:307-15.
24. Khan FA, Akhtar S, Almohazey D, Alomari M, Almofty SA. Extracts of clove Syzygium aromaticum potentiate FMSP nanoparticles induced cell death in MCF-7 cells. Int J Biomater 2018 23;2018:1-10.
25. Meerloo J, Kaspers GJL, Cloos J. Cell sensitivity assays the MTT assay. Cancer cell Culture 2011;2: 237-45.
26. Dorman HJD, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 2000;88:308-16.
27. Verpoorte R, Kim HK, Choi YH. Plants as source for medicines new perspectives. Frontis 2006;261-73.
28. Behbahani BA, Noshad M, Falah F. Study of chemical structure, antimicrobial, cytotoxic and mechanism of action of Syzygium aromaticum essential oil on foodborne pathogens. Potravin Slovak J Food Sci 2019;13:875-83.
29. Azim MHM. Anti tumor, antioxidant and antimicrobial and the phenolic constituents of clove flower buds Syzygium aromaticum. J Microb Biochem Technol 2014; 1:7-8.
30. Kubatka P, Uramova S, Kello M, Kajo K, Kruzliak P, Mojzis J, et al. Antineoplastic effects of clove buds Syzygium aromaticum L) in the model of breast carcinoma. J Cell Mol Med 2017;21:2837-51.
31. Kumar PS, Febriyanti RM, Sofyan FF, Luftimas DE, Abdulah R. Anticancer potential of Syzygium aromaticum L. in MCF-7 human breast cancer cell lines. Pharmacogn Res 2014;6:350-4. A
32. Shahbazi Y. Antioxidant, antibacterial, and antifungal properties of nanoemulsion of clove essential oil. Nanomedicine Res J2019;4:204-8.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

Ethics code: IR.IAU.MSHD.REC.1398



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Vafayi Malek A, Karimi E, Oskoueian E. Comparison of Antioxidant, Antibacterial, and Cytotoxic Effects of Essential Oil and Nanoemulsion of Clove Essential Oil. J. Ilam Uni. Med. Sci. 2021; 29 (3) :26-37
URL: http://sjimu.medilam.ac.ir/article-1-6680-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 29, Issue 3 (8-2021) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 40 queries by YEKTAWEB 4643