fa فراوانی و ارتباط 8111989rs SNP در ژن CKMM A/G مردان وزنه‌بردار و مردان غیرورزشکار تربیت بدنی Physical Education پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar="">مقدمه</span></span></b><span lang="AR-SA" style="font-size:9.0pt"><span style="background:white"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">: </span></span></span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">عوامل ژنتیکی بر عملکرد بدنی، پاسخ تمرین و همچنین وضعیت ورزشی نخبگان تأثیر می‌گذارند؛ بنابراین، تحقیق حاضر با هدف بررسی فراوانی و ارتباط 8111989</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">rs</span></span></span> <span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">&nbsp;SNP&nbsp;</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">در ژن </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">&nbsp;CKMM A/G</span></span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> مردان وزنه‌بردار و مردان غیرورزشکار طراحی شد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:.1pt">مواد و روش ها:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">در این مطالعۀ تحلیلی_توصیفی، 30 نفر از مردان بزرگ‌سال نخبۀ وزنه‌بردار (شامل قهرمانان لیگ برتر، اردونشینان تیم ملی، قهرمانان آسیا، جهان و المپیک) استان اصفهان با میانگین سن 11/7</span></span></span><span lang="FA" style="font-size:9.0pt"><span cambria="" style="font-family:"><span style="letter-spacing:.1pt">&plusmn;</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">77/21، قد 98/6</span></span></span><span lang="FA" style="font-size:9.0pt"><span cambria="" style="font-family:"><span style="letter-spacing:.1pt">&plusmn;</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">87/179 و وزن 73/22</span></span></span><span lang="FA" style="font-size:9.0pt"><span cambria="" style="font-family:"><span style="letter-spacing:.1pt">&plusmn;</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">87/96 (انحراف استاندارد</span></span></span><span lang="FA" style="font-size:9.0pt"><span cambria="" style="font-family:"><span style="letter-spacing:.1pt">&plusmn;</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">میانگین) شرکت کردند که به‌صورت هدفمند، داوطلبانه و در دسترس انتخاب و با تعداد 44 نفر از افراد غیرورزشکار با مشخصه&shy;های مشابه آنتروپومتریکی (سن، قد و وزن) مقایسه شدند. </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">cc</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> 4 نمونۀ بـزاقی از همۀ افراد شرکت‌کننده در این مطالعه جمـع‌آوری گردید و پس از انجام مراحل استخراج </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">DNA</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">، با انجام </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">PCR</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">، شیوع آللی و ژنوتیپی ژن </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">CKMM A/G</span></span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> از طریق روش </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">RFLP</span></span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> و الکتروفورز تعیین شد. برای آزمون فرضیه‌های مربوطه از روش‌های تی &shy;مستقل، کای &shy;اسکوئر و همچنین رگرسیون لجستیک به کمک نرم&shy;افزار </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">SPSS vol.20</span></span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> استفاده گردید. سطح معنی&shy;داری نیز </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">0.05</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">></span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">P</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> در نظر گرفته شد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">یافته‌ها:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">نتایج نشان داد که شیوع ژنوتیپ </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">AG</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> به‌طور معنی‌داری، بالاتر از ژنوتیپ‌های </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">AA</span></span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> و </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">GG</span></span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> در گروه‌های وزنه‌بردار، غیرورزشکار و مجموع جمعیت پژوهش بود (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">0.05</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">></span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">P</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">)؛ همچنین چگونگی توزیع ژنوتیپ </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">CKMM A/G</span></span></span><span style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"> با وضعیت وزنه‌برداری ارتباط معنا‌داری نداشت و میان گروه‌های وزنه‌بردار و غیرورزشکاران نیز تفاوت معنی‌داری یافت نشد (</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">0.05</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt"><</span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:.1pt">P</span></span></span><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">).</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="color:black"><span style="letter-spacing:-.1pt">بحث و نتیجه‌گیری:</span></span></span></span></b> <span lang="FA" style="font-size:9.0pt"><span b="" style="font-family:" zar=""><span style="letter-spacing:.1pt">بر اساس نتایج مطالعه، جامعۀ وزنه‌بردار ایرانی منتخب که در گروه نژاد قفقازی قرار می‌گیرند، احتمالاً ژنتیک بالقوه‌ای در اجرای قابلیت‌های استقامتی و نیز قدرتی/ توانی دارند.</span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Introduction:</span></span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">Genetic factors affect physical performance, exercise response, as well as elite athletic status; Therefore, the aim of the present study was the investigation of the Frequency and association of SNP rs 8111989 in CKMM A/G gene in weightlifting men with non-athlete men.</span></span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.1pt">Material & Methods:</span></span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">In this descriptive study, the subjects were include of all (n=30) elite male weightlifters (league champions, members of national team, and the champions of Asian, world and Olympics) from Isfahan province, age: 21.77&plusmn;7.11; Height: 179.87&plusmn;6.98 and weight: 96.87&plusmn;22.73 (mean&plusmn;standard deviation) Which compared with 44 non-athletes with similar anthropometric (age, height and weight) characteristics. 4 cc saliva samples were collected from each subject and After DNA extraction, the allelic and genotypic prevalence of CKMM A/G gene was determined by PCR via RFLP and electrophoresis. Independent t-test, chi-square and logistic regression were used to analyze the data via the SPSS software version 20. Also the significance level was considered at P<0.05.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:-.1pt">Findings:</span></span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The results showed that the prevalence of AG genotype was significantly higher than AA and GG genotypes in weightlifting, non-athlete and total population groups (P <0.05). Also, there was no significant relationship between CKMM A/G genotype and weightlifting status and there was no significant difference between weightlifters and non-athletes (P >0.05).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:9.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="letter-spacing:.1pt">Discussion & Conclusion:</span></span></span></span></b> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The results showed that the selected Iranian weightlifter, which belongs to the Caucasian race group, has potential genetics in endurance abilities as well as strength / power, likely.</span></span></span></span></span><br> &nbsp; چندشکلی تک‌نوکلئوتیدی, وزنه‌برداران نخبه, ژنومیک ورزشی, غیرورزشکاران Single nucleotide polymorphism, Sport Genomic, Elite Male Weightlifters, Non-athletes 112 122 http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-7044-2&slc_lang=fa&sid=1 Fateme Hashemzade ghodaneh فاطمه هاشم زاده قدنه f.hashemzadeh96@gmail.com 9600319475328460045526 9600319475328460045526 No Dept of Exercise Physiology, Faculty of Educational Sciences and Psychology, Sistan and Baluchestan University, Zahedan, Iran گروه تربیت بدنی و علوم ورزشی، دانشگاه سیستان بلوچستان، سیستان و بلوچستان، ایران Mohammad Reza Batavani محمدرضا باتوانی batavani@iut.ac.ir 9600319475328460045527 9600319475328460045527 Yes Center of Physical Education, Isfahan University of Technology, Isfahan 84156-83111, Iran مرکز تربیت بدنی، دانشگاه صنعتی اصفهان، اصفهان 83111-84156، ایران Mohsen Ghofrani محسن غفرانی m_ghofrani2000@ped.usb.ac.ir 9600319475328460045528 9600319475328460045528 No Dept of Exercise Physiology, Faculty of Educational Sciences and Psychology, Sistan and Baluchestan University, Zahedan, Iran گروه تربیت بدنی و علوم ورزشی، دانشگاه سیستان بلوچستان، سیستان و بلوچستان، ایران