TY - JOUR T1 - Data Analysis of Two Dimensional Electrophoresis Gels by Multivariate Statistical Methods TT - آنالیز داده های ژل های الکتروفورز دو بعدی با استفاده از روش های آماری چند متغیره JF - sjimu JO - sjimu VL - 20 IS - 5 UR - http://sjimu.medilam.ac.ir/article-1-947-en.html Y1 - 2013 SP - 216 EP - 224 KW - Two-dimensional gel electrophoresis KW - Multivariate statistical technique KW - Protein N2 - Background: In recent years technology has emerged to describe the protein spots on two-dimensional gel electrophoresis. Numerous gel statistical analysis software has been developed and the impact of these initiatives on promoting proteomics is impressive. Proteomic analysis of massive data sets with having high variable need multivariate methods to provide the simultaneous analysis of multiple variables. In this study, the process of cellular differentiation and aging of stem cells to astrocytes studied by proteomics and gel analysis software and statistical applications were considered. Materials and Methods: Proteome of four groups (stem cells, young astrocytes, moderately differentiated astrocytes and old astrocytes) were analyzed by the software of Progenesis Same Spots. Cluster analysis, principal component analysis and analysis of power were used in the experimental groups. Results and Discussion: In bioinformatics and statistical analysis of two-dimensional gel electrophoresis technique were detected 940 protein spots with significant expression changes (p M3 ER -