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The Survey of Mandatory Cold Swim Stress Insulin in Male Rats on FBS, OGTT and Serum Insulin in Male Rats
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Mr Shahraki *   1, H Mirshekari , Ar Shahraki , S Khamar moghadam , E Shahraki |
| 1- , m_shahrakim@zaums.ac.ir |
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Abstract: (15435 Views) |
| Introduction: Since stress is considered as an important factor which can affect the releasing of various hormones and metabo-lism, the aim of this survey was to evaluate the effect of mandatory cold swim stress on fasting blood sugar(FBS), oral glucose tolerance test(OGTT) and serum insulin in male rats.
Materials & Methods: This experiment was performed on 30 adult (5-7month) Wistar-Albino male rats (200-250 g weight) which were divided into sham control, control and test groups, randomly (n=10 each group). Test group was given mandatory swim stress in a pool for 1-2 minute but control group was put in the empty pool. Sham control did not undergo stressor during the course of the experiment. OGTT was carried for all animals through the intake of 1 gram glucose until 2 days before the end of experiment course. At e end, the animals were anesthetized by diethyl ether and blood samples were prepared. FBS and OGTT were measured by ordinary methods but serum insulin was measured by ELIZA method.
Findings: The findings showed that FBS, OGTT, food and water intake in the test group were significantly increased compared with those of other groups but body weight were significantly decreased compared with those of sham control and control groups.
Discussion & Conclusion: The results sho-wed that mandatory cold swim stress affected FBS, OGTT and food and water intake in male rats. |
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| Keywords: OGTT, insulin, cold swim str-ess |
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Full-Text [PDF 248 kb]
(2710 Downloads)
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Type of Study: Research |
Subject:
physiology
Received: 2013/05/15 | Accepted: 2013/09/1 | Published: 2013/09/1
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