[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations62733583
h-index2719
i10-index18478

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 20, Issue 5 (3-2013) ::
Journal of Ilam University of Medical Sciences 2013, 20(5): 207-215 Back to browse issues page
Smart Hydrogel designed PNIPAm / chitosan by Gamma Irradiation of Biological applications
S Haydari kashl , E Bi azar * 1, A Karimi , A Asef nejad , A Amir khani , M Shahriari nour , F Kazem begi
1- , kia_esm@yahoo.com
Abstract:   (14117 Views)
The abrupt conformational matrix, in which PNIPAM or its modified forms transition of it upon changes at temperatures, may be embedded. It is expected that the change around 32´C has stirred up explorations for in temperature may induce the conformational technological applications. Methods & Materials: Thermo-sensitive hydrogels were prepared by graft copolymerization of chitosan and N-Isopropylacrylamide via gamma radiation. Characterization of hydrogels such as 13C-NMR, DSC analysis and swelling test and cell assessments for harvesting living cell sheet were investigated. 13C-NMR and DSC analysis showed chitosan and NIPAAm monomer were grafted via gamma radiation successfully. Findings: Swelling ratio and curves results administrated hydrophilicity / hydrophobicity of hydrogel that this property is due to presence of PNIPAAm in different temperatures. The hydrogel was tested for harvesting epithelial cells after carrying out cell culture at 37 °C and incubating the confluent cells at 4°C for spontaneous detachment of cell sheet from hydrogel surface without enzyme treatment. Discussion & Conclusion: Cell viability assay results and microscopic observations demonstrated that cells could attach to the hydrogel surface and maintain high viability and proliferation ability. Cell detachment efficiency from the hydrogel was high. These unique properties of the hydrogel would make it a promising support for epithelial cell grafting especially cornea regeneration.
Keywords: Tissue engineering, intelegent polymer temperature response, chitosan, poly N-Isopropylacrylamide
Full-Text [PDF 459 kb]   (3938 Downloads)    
Type of Study: Research | Subject: Medical microbiology
Received: 2013/03/18 | Accepted: 2013/06/16 | Published: 2013/06/16
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

haydari kashl S, bi azar E, karimi A, asef nejad A, amir khani A, shahriari nour M et al . Smart Hydrogel designed PNIPAm / chitosan by Gamma Irradiation of Biological applications. J. Ilam Uni. Med. Sci. 2013; 20 (5) :207-215
URL: http://sjimu.medilam.ac.ir/article-1-945-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 5 (3-2013) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4643