:: Volume 14, Issue 2 (7-2006) ::
sjimu 2006, 14(2): 1-9 Back to browse issues page
Role of Hyaluronan During Foliculogenesis of Mouse
Abstract:   (40695 Views)
Abstract Introduction:Hyaluronan is an extremely long glycosaminoglycan, which at variance with other glycosaminoglycans, is released into the extracellular. Both proteoglycans and hyaluronan influence many aspects of cell behavior by multiple interactions with other molecules.They synthesis change significantly during ovarian follicular development.This study was aimed to recognize of the structure of hyaluronan named glycosaminoglycans and also its alteration in different parts of follicular development during ovarian cycle. Materials & Methods: In this study, 60 mice with race N-MARI (wt 25-30gr) were prepared from Razi Institute. After adaptation of mice to laboratory conditions and reaching the maturity age, They were used in this study. After appearance of pudenda, which indicate ovarian cycle, their ovaries, were removed and changes in hyaluronan were studied using histochemical methods. Using Alcian Blue staining, pH=3.2, the hyaluronan in prepared tissue blocks from ovarian was evaluated. Findings: The observed changes proved that expression of hyaluronan was increased during folliculogenesis up to preovulatory stage. Hyaluronan synthesis increased in zona pellucida, base membrane and inner teca cells during ovarian cycle but it decreased in follicular cells and follicular fluid after antral stage. Conclusion: It can be concluded that expression of hyaluronan is necessary for expansion of cumulus-oopherous complex during foliculogenesis and ovulation, and also for increase of sperm motility during fertilization.
Keywords: Hyaluronan, Development, Ovary, Follicle, Mouse.
Full-Text [PDF 310 kb]   (4706 Downloads)    
Type of Study: Research | Subject: General
Received: 2009/01/28 | Published: 2006/07/15


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Volume 14, Issue 2 (7-2006) Back to browse issues page