:: Volume 28, Issue 3 (8-2020) ::
sjimu 2020, 28(3): 40-52 Back to browse issues page
Effect of Low-level Laser Therapy on Bone Defect Repair in Diabetic and Osteoporotic Rats using the Real-Time PCR Technique
Atarod sadat Mostafavinia 1, Mohammd Bayat2 , Mehdi Ghatresamani3 , Kamran Ghoreishi4
1- Dept of Anatomy, Faculty of Medicine, Islamic Azad University of Medical Sciences, Tehran, Iran , a.mostafavinia@gmail.com
2- Dept of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Dept of Immunology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
4- Dept of Statistics, Faculty of Basic Sciences, University of Qom, Qom, Iran
Abstract:   (214 Views)
Introduction: Bone formation is disturbed in type 1 diabetes followed by changes in the bone microstructure. The most important metabolic disorder in diabetes is osteoporosis, which is characterized by bone loss and bone structure degradation. This study aimed to determine the effect of low-power laser on bone defect repair in the experimental model of diabetes and osteoporosis.
Materials & Methods: A total of 30 four-month-old female Wistar rats weighing 190-220 g were selected and randomly divided into six groups, including 1: non-diabetic control (Co.), 2: non-diabetic laser (L.), 3: diabetic control (Co.D.), 4: diabetic laser (L.D.), 5: diabetic alendronate (A.D.), and 6: diabetic laser + alendronate (L.A.D.). Diabetes was induced in groups 3, 4, 5, and 6. All groups underwent ovariectomy and partial bone defect. In the laser group, a low-level laser (890nm, 80 Hz, 1/5J / cm 2) was radiated to 3 points at the defect location. Tibia bones were collected, and Real-time PCR was performed after a month. The data were analyzed using ANOVA. A p-value less than P<0.05 was considered statistically significant. Ethics code: 13237-91-1-1393-10397
Findings: The t-test showed a significant decrease in tibia bone density in diabetic and osteoporotic rats, compared to the non-diabetic control group. Moreover, analysis of gene expression data (ANOVA, P<0.05) revealed a significant difference between the group of diabetic laser + alendronate and other groups in terms of Runx2 gene expression and Osteocalcin.
Discussions & Conclusions: According to the findings, laser therapy combined with alendronate can accelerate the repair of partial bone defect in the experimental model of diabetes and osteoporosis.
Keywords: Keywords: Type 1 diabetes|osteoporosis|partial bone defect|low level laser|Real-time PCR. ,
Full-Text [PDF 1108 kb]   (84 Downloads)    
Type of Study: Research | Subject: anatomy
Received: 2019/07/9 | Accepted: 2020/06/29 | Published: 2020/08/31

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Volume 28, Issue 3 (8-2020) Back to browse issues page