[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Editorial Board
Aims& Scopes
Journal News
Articles archive::
All Issues
Current Issue
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
Authors Guideline
Call for Papers
Submission Instruction
Review Process
Research Ethics Statements
Ethical issues
Author Complaint Process
For Reviewers::
Reviewers Section
Subscription::
Registration Information
Subscription Form
Contact us::
Contact Information
Contact us
Site Facilities::
Site map
Search contents
FAQ
Top 10 contents
Inform to friends
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations63203627
h-index2719
i10-index18779
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE
..
:: Volume 28, Issue 2 (5-2020) ::
Journal of Ilam University of Medical Sciences 2020, 28(2): 72-82 Back to browse issues page
Virulence Potential of Rhizopus Species in an Embryonated Chicken Egg Model
Somayeh Dolatabadi * 1, Zahra Pourfraidongahasrodashti2 , Mohammad javad Najafzadeh3 , Seyed Abolfazl Hoseyni4
1- Faculty of engineering, Sabzevar University of New Technology, Sabzevar, Iran , somayeh99@gmail.com
2- Dept of Pathology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3- Dept of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Dept of Biology, Faculty of Life Sciences, Hakim Sabzevari University, Sabzevar, Iran
Abstract:   (2308 Views)
Introduction: Little is known about the pathogenicity and virulence properties of the Rhizopus species used in food fermentation. This study aimed to investigate the virulence potential of Rhizopus arrhizus and R. microsporus strains obtained from a wide selection of clinical and environmental sources in an embryonated chicken egg model.
 
Materials & Methods: In total, 26 strains (13 strains from each species) were inoculated in embryonated eggs with final concentration of 106 spore/ml/egg. The eggs were inoculated (0.1 ml) through chorio-allantoic membrane in day 10 and incubated at 37.6 °C and 60% humidity and monitored for 7 days. Ethics code: IR.MUMS.fm.REC.1396.457
Findings: The mortality rate for R. microsporus was slightly higher than that of R. arrhizus. The ability of R. arrhizus and R. microsporus to cause infection was strain-specific but source-independent in the embryonated chicken eggs. The presence of endosymbiont bacteria (Burkhulderia) in some strains of R. microspores did not show any effect on their virulence.
 
Discussion & Conclusions: The occurrence of virulence in strains isolated from fermented food indicates the opportunistic nature of these fungi and possibility of a public health risk for consumers.
Keywords: Fermented food, Embryonated chicken egg model, Mucorales, Rhizopus, Rhizopus, Virulence
Full-Text [PDF 719 kb]   (923 Downloads)    
Type of Study: Research | Subject: mycology
Received: 2019/05/18 | Accepted: 2020/02/1 | Published: 2020/06/30
References
1. Dolatabadi S. Mucorales between food and infection. PhD thesis Amsterdam Uni. 2015.
2. Hesseltine CW. Microbiology of oriental fermented foods. Annu Rev Microbiol 1983; 37: 575–601. doi. 10.1146/annurev.mi.37.100183.003043
3. Jennessen J, Nielsen KF, Houbraken J, Lyhne EK, Schnurer J, Frisvad JC, et al. Secondary metabolite and mycotoxin production by the Rhizopus microsporus group. J Agr Food Chem 2005; 53: 1833–40. doi.10.1021/jf048147n
4. Hong SB, Kim DH, Lee M, Baek SY, Kwon SW, Houbraken J, et al. Zygomycota associated with traditional meju, a fermented soybean starting material for soy sauce and soybean paste. J Microbiol 2012; 50:386-93. doi.10.1007/s12275-012-1437-6
5. Dolatabadi S, Walther G, Gerrits AHG, Hoog GS. Diversity and delimitation of Rhizopus microsporus. Fung Div 2014; 64:145-63. doi.10.1007/s13225-013-0229-6
6. Walther G, Pawłowska J, Izquierdo A, Wrzosek M, Rodriguez JL, Dolatabadi S, Chakrabarti A, et al. DNA barcoding in Mucorales an inventory of biodiversity. Persoonia 2013; 30:11-47. doi. 10.3767/003158513X665070
7. Ibrahim AS, Spellberg B, Walsh TJ, Kontoyiannis DP. Pathogenesis of mucormycosis. Clin Inf Dis 2012;54: 16-22. doi. 10.1093/cid/cir865
8. Rammaert B, Lanternier F, Zahar JR. Healthcare associated mucormycosis. Clin Inf Dis 2012;54: 44-54. doi. 10.1093/cid/cir867
9. Lanternier F, Sun HY, Ribaud P, Singh N, Kontoyiannis DP, Lortholary O. Mucormycosis in organ and stem cell transplant recipients. Clin Inf Dis 2012;54: 35-43.
10. Binder U, Maurer E, Lass C. Mucormycosis from the pathogens to the disease. Clin Microbiol Inf 2014; 20:6:60-6. doi.10.1093/cid/cis195
11. Kontoyiannis DP, Marr KA, Park BJ, Alexander BD, Anaissie EJ, Walsh TJ. Prospective surveillance for invasive fungal infections in hematopoietic stem cell transplant recipients 2001-06 overview of the transplant associated infection surveillance network database. Clin Inf Dis 2010; 50:1091-100. doi. 10.1086/651263
12. Rohm B, Scherlach K, Mobius N, Partida LP, Hertweck C. Toxin production by bacterial endosymbionts of a Rhizopus microsporus strain used for tempe sufu processing. Int J Food Microbiol 2010; 136:368-71. doi. 10.1016/j.ijfoodmicro.2009.10.010
13. Dolatabadi S, Hoog GS, Meis JF, Walther G. Species boundaries and nomenclature of Rhizopus arrhizus. Mycoses 2014b; 57: doi. 10.1111/myc.12228.
14. Jacobsen ID, Grobe k, Berndt A, Hube B. Pathogenesis of Candida albicans infections in the alternative chorio allantoic membrane chicken embryo model resembles systemic murine infections. PLos One 2011;6: 19741. doi. 10.1371/journal.pone.0019741
15. Hartl A, Hillesheim HG, Kunkel W, Schrinner EJ. The Candida infected hens egg an alternative test system for systemic anticandida activity. Arzne Im Sch1995; 45:926-8.
16. Skiada A, Pagano L, Groll A. Analysis of 230 cases accrued by the registry of the European confederation of medical mycology working group on Zygomycosis between 2005 and 2007. Clin Microbiol Inf2011; 17:1859-67. doi. 10.1111/j.1469-0691.2010. 03456.x
17. Alvarez E, Sutton DA, Cano J, Fothergill AW, Stchigel A, Rinaldi MG, et al. Spectrum of zygomycete species identified from clinically significant specimens in the United States. J Clin Microbiol 2009; 47:1650–1656. doi. 10.1128/JCM.00036-09
18. Wanga JW, Zhoua JY, Yanga QW. An improved embryonated chicken egg model for the evaluation of antiviral drugs against influenza A virus. J Virol Meth 2008; 153:218-222. doi. 10.1016/j.jviromet.2008.06.022
19. Jander G, Rahme LG, Ausubel FM. Positive correlation between virulence of Pseudomonas aeruginosa mutants in mice and insects. J Bacteriol 2000; 182:3843-5. doi. 10.1128/jb.182.13.3843-3845.2000
20. Anderson C, Gripenland J, Johansson J. Using the chicken embryo to assess virulence of Listeria monocytogenes and to model other microbial infections. Nat Prot 2015; 10:1155-64. doi. 10.1038/nprot.2015.073
21. Jacobsen ID, Grobe K, Slesiona S, Hube B, Berndt A, Brock M. Embryonated eggs as an alternative infection model to investigate Aspergillus fumigatus virulence. Inf Immun 2010; 28:2995-3006. doi. 10.1128/IAI.00268-10
22. Jacobsen ID, Grobe K, Slesiona S, Hube B, Berndt A, Brock M. Embryonated eggs as an alternative infection model to investigate Aspergillus fumigatus virulence. Inf Immun 2010; 78:2995-3006. doi. 10.1128/IAI.00268-10
23. Kaerger K, Schwartze VU, Dolatabadi S, Nyilasi I, Kovas SA, Binder U, et al. Adaptation to thermotolerance in Rhizopus coincides with virulence as revealed by avian and invertebrate infection models, phylogeny physiological and metabolic flexibility. Virulence 2015;6:395–403. doi. 10.1080/21505594.2015.1029219
24. Schwartze VU, Hoffmann K, Nyilasi I, Papp T, Vagvolgyi C, Hoog S, et al. Lichtheimia species exhibit differences in virulence potential. Plos One 2012;7: 40908. doi. 10.1371/journal.pone.0040908
25. Ibrahim AS, Gebremariam T, Liu M, Chamilos G, Kontoyiannis DP, Mink R, et al. Bacterial endosymbiosis is widely present among zygomycetes but does not contribute to the pathogenesis of mucormycosis. J Inf Dis 2008; 198:1083-90. doi: 10.1086/591461
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Dolatabadi S, Pourfraidongahasrodashti Z, Najafzadeh M J, Hoseyni S A. Virulence Potential of Rhizopus Species in an Embryonated Chicken Egg Model. J. Ilam Uni. Med. Sci. 2020; 28 (2) :72-82
URL: http://sjimu.medilam.ac.ir/article-1-5686-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 2 (5-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 41 queries by YEKTAWEB 4646