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Prodution of Polyclonal Antibody Against Giardia Lamblia in Rabbit
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Abstract: (19365 Views) |
| Abstract
Introduction: Giardia lamblia is the most frequently human intestinal protozoan in the worldwide. Diagnosis of G. lamblia by microscopic examination of stool is common. ELISA& IFA methods have researching and epidemiological use. For designing a powerful test with high detection rate like dipstick or rapid diagnosis kits first step is production of polyclonal antibody. For this reason we decided to produce polyclonal antibody against G. lamblia in rabbit.
Material and methods: Giardia cysts were purified from human fecal samples by sucrose two stage modified gradient method. Their concentration was independently adjusted to 1× 106/ml PBS, were inoculated intramasculary-intradermaly in four stage with freunds complete adjuvant for first time and with freunds uncomplete adjuvant for second time and without freunds adjuvant for next time, in rabbit. After immunization of rabbit, IFA test was carried out to evaluate the polyclonal antibody production against Giardia.
Results: The results of test showed the rabbit was immuned. Best results of IFA were achieved with 1×106/ml parasite and sera dilution of 1/100 and goat anti rabbit IgG conjugated with FITC dilution of 1/20 is under immunofloursent microscope.
Conclusion: Giardias antigens have an important role for diagnosis in clinical and other samples. Production of anti Giardia polyclonal antibody with high titer is first and important step for designing IFA and direct ELISA kits for direct detection of Giardias antigens. Produced polyclonal antibody can use purification and conjugation with enzyme. |
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| Keywords: Production, Giardia lamblia, Polyclonal antibody |
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Full-Text [PDF 570 kb]
(8528 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2009/01/27 | Accepted: 2015/04/21 | Published: 2015/04/21
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