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Genetic Diversity of Proteus Mirabilis Isolated
from Urine Specimens by Box PCR
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Soheila Rezvan1    , Kumarss Amini * 2 |
1- Dept of Microbiology, Faculty of Basic Sciences, Sirjan Branch, Islamic Azad University, Sirjan, Iran
2- Dept of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , dr_kumarss_amini@yahoo.com |
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Abstract: (3428 Views) |
Introduction: Proteus mirabilis is one of the common causes of UTI.Multiple molecular methods are used to genotype bacteria.The polymerase chain reaction method is based on repeated sequences using BOXA1R primers for differentiation of bacteria. This study aimed to evaluate the genetic diversity of protease mirabilis isolated from urine specimens.
Materials & Methods: In this descriptive cross-sectional study, 60 isolates protease mirabilis were identified from urine specimens using biochemical tests. DNA samples were extracted and tested for BOX-PCR.
Findings: The tree diagram or dendrogram of BOX-PCR was drawn and the results showed that all strains were segregated into 7 separate clusters at a similarity level of 57%. There were three isolates in the first group, nine in the second group, 18 in the third group, two in the fourth group, 11 in the fifth group, one in the sixth group, and 16 isolates in the seventh group. Dandrogram analysis revealed that the strains belonging to a serovar are subtracted from each other.
Discussion & Conclusions: This method demonstrated that molecular fingerprinting with BOXA1R primers is useful for typing bacterial isolates from different origins. Moreover, it has a really efficient differentiation power, it can be used effectively in epidemiological studies, and trace source of infection and taxonomy.
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| Keywords: Proteus mirabilis, BOX-PCR, Genotyping |
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Full-Text [PDF 740 kb]
(1378 Downloads)
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Type of Study: Research |
Subject:
Medical microbiology
Received: 2017/12/19 | Accepted: 2018/08/27 | Published: 2019/09/15
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