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:: Volume 26, Issue 3 (9-2018) ::
sjimu 2018, 26(3): 169-176 Back to browse issues page
Presence of anti-glucose regulated protein78 autoantibodies in the sera of women with recurrent pregnancy loss
Behrooz Gharesifard 1, Mohamad Hosein Karimi 2, Leila Bidar 3, Mohamad Ali Hassanzadeh 4
1- Dept of Immunology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran , gharesifb@sums.ac.ir
2- Organ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3- Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Dept of Immunology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (165 Views)
Introduction: Recurrent pregnancy loss (RPL) is defined as three or more miscarriages prior to the 20th week of gestation. This condition occurs in 15-20% of all pregnancies. Glucose-regulated protein 78 (GRP78) is an endoplasmic reticulum (ER) protein that is expressed on the trophoblast cells. The purpose of this study was to evaluate the presence of anti-GRP78 antibodies in the sera of women with RPL.
 
Materials & Methods: This case-control study was conducted 80 women referring to the Infertility Center of Shiraz University of Medical Sciences, Shiraz, Iran, during 2014-2016. The study population was assigned into two groups of case entailing 38 women with unexplained RPL and control including 42 healthy pregnant women with no history of miscarriage and a minimum of two successful pregnancies. The measurement of the anti-GRP78 antibody in the sera of the two groups was performed using the enzyme-linked immunosorbent assay (ELISA). Furthermore, western blot technique was used to assess the expression of GRP78 in placenta tissue for both groups.
 
Findings: The results of the western blot demonstrated the expression of GRP78 on the trophoblast cells in both groups. Furthermore, anti-GRP78 antibodies were detected in both case and control groups through ELISA. The case group had a lower level of anti-GRP78 antibodies; however, this difference was not statistically significant (P=0.1).
 
Discussion & Conclusions: The findings of the current study revealed no significant difference [Zamen1] between anti-GRP78 antibody level in RPL patients and healthy pregnant subjects.
 

Keywords: pregnancy, RPL, placenta, GRP78
Full-Text [PDF 658 kb]   (43 Downloads)    
Type of Study: Applicable | Subject: immunology
Received: 2017/10/26 | Accepted: 2018/02/5
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Gharesifard B, Karimi M H, Bidar L, Ali Hassanzadeh M. Presence of anti-glucose regulated protein78 autoantibodies in the sera of women with recurrent pregnancy loss . sjimu. 2018; 26 (3) :169-176
URL: http://sjimu.medilam.ac.ir/article-1-4606-en.html


Volume 26, Issue 3 (9-2018) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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