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The Prevalence of Antibiotic Resistance in Methicillin-Resistant Staphylococcus aureus and the Determination of Aminoglycoside Resistance Gene aac(6΄)-Ie/aph (2˝) Isolated from Hospitalized Patientsin Imam Hossein, Loghman Hakim,
and Pars Hospitals in Tehran using
Polymerase chain Reaction
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Mina Kavusi1    , Fahimeh Nemati Mansour * 2, Seyed Mohsen Mahdion3 |
1- Dept of Microbiology, Faculty of Advanced Sciences and Technology, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
2- Dept of Biotechnology, Faculty of Advanced Sciences and Technology, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran , mohsen.mahdiyoun@yahoo.com
3- Dept of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran |
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Abstract: (5037 Views) |
Introduction: Staphylococcus aureus is one of the critical pathogens resulted in hospital land community-acquired infections. Aminoglycosides are potent bactericidal agents that are often used in staphylococcal infection treatment in combination with a beta-lactam or glycopeptide antibiotics. The aim of this study was to determine the frequency of encoding gene ofaac (6΄)-Ie/aph (2˝).This gene is one of the important aminoglycoside modifying enzymes in combination with mecA which results in methicillin resistance. In doing so, disk diffusion and polymerase chain reaction (PCR) methods were utilized in clinical isolates of methicillin resistant staphylococcus aureus (MRSA).
Materials & Methods: In the current study, 174 clinical isolates of MRSA were obtained from different clinical specimens, including blood, sputum, trachea, brunch, pleura, urine, wound, and catheter. Antibiotic resistance of erythromycin, clindamycin, ciprofloxacin, gentamicin, cefazolin, rifampicin, doxycycline, cotrimoxazole and vancomycin was determined using Kirby-Bauer disk diffusion test method according to the CLSI guidelines. The presence of MRSA was confirmed using oxacillin and cefoxitin anti biotic disc diffusion. Subsequently, DNA of MRSA isolates was investigated to detect mecA and aminoglycoside resistance aac (6΄)-Ie/aph (2˝) genes using PCR.
Findings: The results obtained from biogram anti-microbial susceptibility test system indicated that all of the isolates were resistant to oxacillin and cefoxitin. All of the isolates were sensitive to vancomycin and majority of them were resistant to erythromycin (84.8%). According to PCR test results, 100 and 78.3% of the isolates were positive for the mecA and aac(6′)/aph(2˝)-Ia genes, respectively.
Discussion & Conclusions: According to the results, the aminoglycoside resistance geneswere highly prevalent in MRSA isolates. |
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| Keywords: aac(6΄)-Ie/aph(2˝), Aminoglycoside modifying enzyme, mecA, Methicillin resistant Staphylococcus aureus |
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Full-Text [PDF 759 kb]
(3613 Downloads)
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Type of Study: Research |
Received: 2017/06/15 | Accepted: 2018/02/12 | Published: 2019/04/15
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