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:: Volume 23, Issue 5 (11-2015) ::
sjimu 2015, 23(5): 173-181 Back to browse issues page
The Evaluation ofAntimicrobial Properties of Zink and Silver Nanoparticles on Pathogenic BacteriaPseudomonas aeruginosa and Staphylococcus Aureus
Yasaman Nabipour1, Arman Rostamzad 2, Salman Ahmadi3
1- Ilam University
2- Ilam University , a.rostamzad381@yahoo.com
3- Mazandaran University
Abstract:   (7492 Views)

Introduction: In the current  research, the effectiveness of silver's Nano- particles anti- microbial activity on the pathogenic and resistant positive- gram and negative-gram bacteria in the hospital infections have been studied. In this study, the primary density of bacteria was constant and only Nano- particles and items of studied bacteria were variable.

Materials & methods: At first, this study uses the available chemical reagents and conditions only apply controlling optimum conditions, nanoparticles with a size of about 20-35 nm were synthesized by chemical precipitation method. Using Macro dilution  method the different densities of silver Nano- particles and Zinc Nano- particles (medium+ Nano- particle) were prepared as: 0.01 percent, 0.1 percent, 0.5 percent, 1 percent and 1.5 percent. Then density of 1.5 cell/ml was prepared for Staphylococcus aureus Pseudomonas aeruginosa the containers which included treatment medium (bacterium + Nano- particle) and control medium have been placed in incubator shaker with 250 cycle in minute and 37˚C for 24h. after passing this time, light density 600 nm ware length was used for measuring bacteria density, then OD in treatment and + control and – control were determined. Nano- particles dilutions were used in bacteria medium for calibrating spectrophotometer as a blank solution. . These results are statistically significant. (P-value <0.01).

Findings: Statistical analysis about Zn Ag Nano- particles showed that in 0.5 percent density Nano- particles were bactericide and they could eliminate 100% of Staphylococcus aureusPseudomonasaeruginosa and in 0.01 percent density for all two bacteria, these Nano- particles were bacteriostatic.

Discussion & Conclusion: in the laboratorial tests of Nano-Materials, contact with them, bacteria will be killed. The obtained results for Nano- particles' anti- bacteria property showed that there is a direct relationship between Nano- particles density and the amount of bacteriums elimination.

Keywords: Nano- particles, Macro dilution method, Incubator shaker, Bacteriostatic, Bactericide
Full-Text [PDF 625 kb]   (8320 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2014/06/8 | Accepted: 2015/01/24 | Published: 2015/11/29
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Nabipour Y, Rostamzad A, Ahmadi S. The Evaluation ofAntimicrobial Properties of Zink and Silver Nanoparticles on Pathogenic BacteriaPseudomonas aeruginosa and Staphylococcus Aureus. sjimu. 2015; 23 (5) :173-181
URL: http://sjimu.medilam.ac.ir/article-1-1869-en.html


Volume 23, Issue 5 (11-2015) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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