:: Volume 17, Issue 3 (10-2009) ::
sjimu 2009, 17(3): 10-15 Back to browse issues page
Effects of Micro-filter in Detection of Coliform in Tap Water by PCR
Hamid Abtahi
Abstract:   (33330 Views)
Introduction: For detection of E.coli in top water by PCR, the samples must be concentrated by filter. In this study, we investigated two kinds of micro filters in PCR results. Materials & Methods: After preparing dilution of E.coli (8/100, 4/100, 2/100, 1/100, 1/200, 1/400, 1/800 and 1/1600) in D.W, the bacteria were separated by FHLP and HAWP filters and 16s rRNA was propagated by the above- mentioned primers. Findings: The rate of bacteria in dilutions was confirmed by culture. The PCR data showed that FHLP, better than HAWP filter, would be able to separate the bacteria in such dilutions after filtration. Discussion & Conclusion: The results of this study showed that hydrophobic filter (FHLP) has a higher ability than that of hydrophilic filter (HAWP) in separating bacteria. So, successful PCR amplifications were achieved by cells concentrated with hydrophobic filters for detection of all the coliform bacteria, while false negative results decreased considerably.
Keywords: coliform, PCR, microfilter
Full-Text [PDF 296 kb]   (3586 Downloads)    
Type of Study: Research | Subject: General
Received: 2010/02/9 | Accepted: 2015/04/20 | Published: 2015/04/20

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Volume 17, Issue 3 (10-2009) Back to browse issues page