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:: Volume 25, Issue 3 (9-2017) ::
sjimu 2017, 25(3): 12-22 Back to browse issues page
Evaluation of the Effect of Mesenchymal Stem Cells pulsed with Caffeine on the Function of the Peritoneal Macrophages of Rat
Nazanin Shushtari 1, Meysam Abtahi Froushani Seyyed 2
1- DVM Dept of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2- Assistant Professor Dept of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , meysamabtahi@hotmail.com
Abstract:   (899 Views)

Introduction: Pervious studies indicated that the mesenchymal stem cells (MSCs) produce adenosine and express adenosine receptors. Moreover, produced adenosine has biological effect on these cells. On the other hand, MSCs have a wide relationship with macrophages in tissues and bone marrow. The aim of the present study was to determine the effects of the MSCs treated with caffeine, as an adenosine antagonist, on the peritoneal macrophages isolated from rats.
 
Materials & methods: After isolation of mesenchymal stem cells from bone marrow of rats, these cells pulsed with different concentration of caffeine (0, 0.1, 0.5 and 1 mM) for 48h. Then, MSCs were co-cultured with macrophages for 1 day. Then, the level of nitric oxide production and respiratory burst of macrophages after challenge with N-formylmethionine-leucyl-phenylalanine (f-MLP) or opsonized yeast were evaluated.
Findings: It seems that MSCs pulsed with caffeine or without it could significantly decrease the respiratory burst and nitric oxide production of co-cultured macrophages more prominent than macrophages alone after unspecific challenged with fMLP. Although, pervious treatment of MSCs with caffeine could decrease the respiratory burst and nitric oxide production of macrophages challenged with f-MLP more prominent than MSCs without treatment. However, the potential of nitric oxide production and respiratory burst of various group after challenge with opsonized yeast didn’t show any significant differences.
 
Discussion & conclusions: Collectively, these data may offer new insight into the potential mechanisms underlying the immunomodulatory and anti-inflammatory effects of caffeine.

Keywords: Mesenchymal Stem Cell, Macrophage, Caffeine
Full-Text [PDF 749 kb]   (270 Downloads)    
Type of Study: Research | Subject: immunology
Received: 2015/12/10 | Accepted: 2016/06/11 | Published: 2017/08/28
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Shushtari N, Abtahi Froushani M. Evaluation of the Effect of Mesenchymal Stem Cells pulsed with Caffeine on the Function of the Peritoneal Macrophages of Rat. sjimu. 2017; 25 (3) :12-22
URL: http://sjimu.medilam.ac.ir/article-1-3247-en.html


Volume 25, Issue 3 (9-2017) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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